Detection Contaminant DNA of a Pig on a Sample Meat Mills in Some City Markets Bengkulu, Using a Genetic Marker DNA Mitochondria Cytochrome B

Abstract

The purpose of the study was to find out if there was a mixture of pork in ground beef samples in some Bengkulu city markets using the molecular Genetic DNA mitochondrial cytochrome b. The research was conducted from December 2019 to May 2020. Sampling was conducted at 10 meat mills in Bengkulu City (Pasar Panorama, Pasar Minggu, Pasar Barukoto, and Sungai Serut Fish Market). DNA isolation, DNA amplification, and electrophoresis are carried out in the biotechnology laboratory, Department of Biology, University of Bengkulu. The meat samples that had been milled were then performed genome DNA isolation following the Qiagen DNeasy for Food Kit protocol. PCR (Polymerous Chain Reaction) is carried out as many as 30 cycles with Primer Forward referring to and primary Reverse Mix (pork, cow, and chicken), Reverse Pork for pigs and Reverse cow for cows. PCR products are electrophobic on 1% agarose gel and then visualized under UV light using Gel Document System Axygen. As a result of the study, on the primary use of Rcow all samples contained cow DNA. In primary use reverse pork, no DNA tape is amplified. In the primary use of Reverse mix from ten samples all containing cow DNA, one sample is thought to contain pig DNA i.e. s7 samples, and six of them contain chicken DNA (S1, S4, S5, S6, S7, and S8). Generally, meat milling in Bengkulu city located in Pasar Panorama, Pasar Minggu, Pasar Barukoto, and Sungai Serut Fish Market is not tainted with pork even though one sample is found, this is because it is influenced by the amount of meat that is milled still too much.