The Effect of Piperine on MMP-9, VEGF, and E-cadherin Expression in Breast Cancer MCF-7 Cell Line

Z. Zare, Tina Nayerpour dizaj, Armaghan Lohrasbi, Zakieh Sadat Sheikhalishahi, Mohammad Panji, F. Hosseinabadi, Vajiheh Najafi, Omid Abazari, M. Abbasi, Parisa Khanicheragh
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引用次数: 3

Abstract

Background: Vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and E-cadherin play a vital role in the behavior of angiogenesis, metastasis, and invasion of breast tumor cells. Piperine, the main component of Piper Nigrum, has shown anti-cancer properties in various malignancies. This Study investigates the potential effect of piperine on MMP-9, E-cadherin, and VEGF expression in breast cancer MCF-7 cell line. Methods: MTT assay was applied to assess the viability of MCF-7 cells. The mRNA levels of MMP-9, VEGF, and E-cadherin were assayed by qRT-PCR. Western blot was performed to identify the protein level of MMP-9. Results: MTT assay results showed that piperine treatment (5, 10, 25, 50, 75, and 100 μM) for 24 hours effectively inhibited cell viability of MCF-7 cells as compared with the control group. Furthermore, the gene expression of VEGF, MMP-9, and E-cadherin was dose-dependently suppressed by piperine treatment (5, 10 and 25 μM) (P<0.05; P<0.01). The results also indicated that piperine (5, 10, and 25 μM) significantly suppressed MMP-9 protein expression after 24 hours of piperine treatment (P<0.01). Conclusion: These results suggest that piperine may prevent angiogenesis, migration, and invasion of MCF-7 cells by suppressing MMP-9 and VEGF, and by inducing E-cadherin expression. Hence, it may be a suitable candidate for designing new drugs in cancer therapy.
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胡椒碱对乳腺癌MCF-7细胞系中MMP-9、VEGF和E-cadherin表达的影响
背景:血管内皮生长因子(VEGF)、基质金属蛋白酶-9 (MMP-9)和E-cadherin在乳腺肿瘤细胞的血管生成、转移和侵袭行为中起重要作用。胡椒碱是胡椒的主要成分,对多种恶性肿瘤具有抗癌作用。本研究探讨胡椒碱对乳腺癌MCF-7细胞系中MMP-9、E-cadherin和VEGF表达的潜在影响。方法:采用MTT法测定MCF-7细胞活力。采用qRT-PCR检测MMP-9、VEGF、E-cadherin mRNA表达水平。Western blot检测MMP-9蛋白表达水平。结果:MTT实验结果显示,胡椒碱(5、10、25、50、75和100 μM)处理24h后,MCF-7细胞活力明显受到抑制。此外,胡椒碱处理(5、10和25 μM)对VEGF、MMP-9和E-cadherin的基因表达呈剂量依赖性抑制(P<0.05;P < 0.01)。胡椒碱(5 μM、10 μM和25 μM)对MMP-9蛋白的表达有显著抑制作用(P<0.01)。结论:胡椒碱可能通过抑制MMP-9、VEGF及诱导E-cadherin表达抑制MCF-7细胞的血管生成、迁移和侵袭。因此,它可能是设计癌症治疗新药的合适候选者。
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审稿时长
12 weeks
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