{"title":"Inhibitory Mechanism of Camellia oleifera Shell Extract on Tyrosinase","authors":"璐 王","doi":"10.12677/hjfns.2022.112018","DOIUrl":null,"url":null,"abstract":"Objective: To explore the inhibitory effect of Camellia oleifera shell extract on tyrosinase, to determine the type of inhibition, to preliminarily explore the inhibition mechanism, and to determine its active components. Method: Using L-tyrosine and L-DOPA as substrates, respectively, the inhibitory effects of Camellia oleifera shell extract on tyrosinase monophenolase activity and diphenolase activity were determined. The Lineweaver-Burk double-reciprocal plot method was used to explore the inhibition mechanism, and the contents of total polyphenols and flavonoids in Camellia oleifera","PeriodicalId":12938,"journal":{"name":"Hans Journal of Food and Nutrition Science","volume":"8 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hans Journal of Food and Nutrition Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12677/hjfns.2022.112018","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To explore the inhibitory effect of Camellia oleifera shell extract on tyrosinase, to determine the type of inhibition, to preliminarily explore the inhibition mechanism, and to determine its active components. Method: Using L-tyrosine and L-DOPA as substrates, respectively, the inhibitory effects of Camellia oleifera shell extract on tyrosinase monophenolase activity and diphenolase activity were determined. The Lineweaver-Burk double-reciprocal plot method was used to explore the inhibition mechanism, and the contents of total polyphenols and flavonoids in Camellia oleifera