Defence response of local rice variety Acuce in Hani terraced fields in Yunnan against the feeding of Sogatella furcifera

Abstract

[Objective] To study the main defensive response of Acuce, a local rice variety in the Hani terraced fields in Yuanyang, Yunnan under the feeding stress of white-backed planthopper (WBPH), and to clarify its WBPH resistance mechanism. To provide a basis for rice insect-resistant breeding and utilization of insect-resistant germplasm resources. [Method] The contents of nutrient soluble sugar, secondary products malondialdehyde (MDA) and hydrogen peroxide (H 2O 2) in rice plants were determined by colorimetric method at 0, 3, 6, 12, 24, 48, 72 and 96 h after WBPH feeding on Acuce, insect susceptible variety Taichung Native 1 (TN1) and insect resistant variety Rathu Heenati (RHT) in the meantime, the main defense reaction of main protective enzyme activity including peroxidase (POD), phenylalanine ammonia-lyase (PAL) and catalase (CAT) were measured and analyzed. [Result] After WBPH feeding stress 3-96 h, the soluble sugar content of the Acuce increased by 93.19%. The increase rate was significantly lower than TN1(176.23%) ( P<0.05, the same as below), there was no significant difference with RHT(89.16%) ( P>0.05, the same as below). The increase rate of H 2O 2 content was 44.13%, significantly higher than TN1(13.18%) and significantly lower than RHT(83.01%). The content of MDA in the rice plant of Acuce increased, and the mean increase rate was 145.38%, significantly higher than that of TNI(36.84%) and RHT(57.06%). The activities of POD, CAT and PAL were increased after feeding stress. The mean increase rate of POD activity (52.78%) was not significantly different with TN1(52.30%), but was significantly lower than that of RHT(154.53%). The mean increase rate of CAT activity (26.24%) was significantly higher than that of RHT(-9.32%) and TN1(-14.18%). The mean increase rate of PAL activity (3.80%) was significantly higher than that of RHT(-32.01%) and significantly lower than that of TN1(74.03%). [Conclusion] After WBPH feeding, the local rice variety Acuce is more sensitive in nutrients, secondary substances, and major protective enzymes than the insect susceptible variety TN1 and resistant variety RHT, showing a strong defense response. Acuce can be used as the WBPH resistance varieties in the field. 摘要: 【目的】 研究云南元阳哈尼梯山水稻地方品种月亮谷对白背飞虱取食胁迫的主要防御反应,明确月亮谷抗 白背飞虱机制,为水稻抗虫育种及抗虫种质资源利用提供科学依据。 【方法】 以元阳地方水稻品种月亮谷、感虫水稻品 种Taichung Native 1 (TN1) 和抗虫品种Rathu Heenati(RHT)为材料, 采用比色法测定3个水稻品种在白背飞虱取食胁 迫0、3、6、12、24、48、72和96 h后稻株内营养物质可溶性糖、次生产物丙二醛(MDA)和过氧化氢(H 2O)含量及保护酶 过氧化物酶(POD)、苯内'氨酸解氨酶(PAL)和过氧化氢酶(CAT)活性。 【结果】 白背飞虱取食胁迫3~96 h后, 月亮谷稻 株内可溶性糖平均含量增加率为93.19%, 显著小于TN1(176.23%) ( P<0.05,下同), 而与RHT(89.16%)无显著差异( P>0.05,下同)；H 2O 2平均含量增加率为44.13%, 显著高于TN1(13.18%), 而显著低于RHT(83.01%);MDA平均含量增加 率为145.38%, 显著高于TN1 (36.84%)和RHT(57.06%); POD、CAT和PAL活性均增加, 其中POD活性平均增加率 (52.78%)与TN1 (52.30%)无显著差异而显著低于RHT (154.53%), CAT活性平均增加率(26.24%)均显著高于RHT (-9.32%)和TN1(-14.18%), PAL活性平均增加率(3.80%)显著高于RHT(-32.01%)而显著低于TN1(74.03%)。 【结论】 月亮谷受白背飞虱取食胁迫后, 其营养物质、次生产物及主要保护酶活性均较感虫品种TN1和抗虫品种RHT反应敏 感, 表现出强烈的防御反应, 月亮谷可作为抗白背飞虱品种在山间应用。