Green synthesis of silver nanoparticles by Aspergillus consortium and evaluating its anticancer activity against Human breast adenocarcinoma cell line (MCF7)

P. Rajeswari, P. Samuel, J. Vijayakumar, T. Selvarathinam, Sudarmani D.N.P., K. Amirtharaj, R. Deenathayalan
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引用次数: 10

Abstract

Objective : The objective of the present study is to produce silver nanoparticles from marine fungi from south west coastal areas of Tamil Nadu and evaluating their potentials with special reference to anticancer activity. Methods : Hand core pushing technique is adapted to collect marine sediment samples along the coastal environs. Distinguished Aspergillus colonies were isolated and identified by wet mount procedure. The characterized Aspergillus consortium was subjected to produce silver nanoparticles. The extracellularly synthesized nanoparticles were characterized. Silver nanoparticles were evaluated for anticancer activity against MCF7 cell line by MTT assay. The IC 50 values were determined. Results : Aspergillus consortium consist of A. niger, A. michelle and A. japonicus . Silver nanoparticles were extracellularly synthesized by the reduction of silver nitrate (AgNO 3 ) to metallic silver (Ag + ) ions results in the transformation of pale yellow to dark red colour. Constant shift was observed at 420 nm while monitoring the solution by UV-Vis spectrophotometer. The presence of important functional groups like –NH 3 was confirmed by FT-IR Spectroscopy. Anticancer activity of the silver nanoparticles was evaluated against MCF7. There was 100% cell inhibition when concentration of the AgNPs reached 25 µg, 50 µg and 100 µg respectively in the test solution. Notably the IC 50 value was found to be very lowest for the nanoparticles produced by A. japonicus and the value was found to be 1.47 µg/ml. Conclusions : Aspergillus consortium was found to be an ideal mycobiosystem for the production of silver nanoparticles with potential anticancer activity.
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曲霉绿色合成纳米银及其对人乳腺腺癌细胞(MCF7)的抗癌作用
目的:从泰米尔纳德邦西南沿海地区的海生真菌中制备纳米银,并评价其抗癌活性。方法:采用手推岩心法采集沿海地区海相沉积物样品。采用湿法分离鉴定了曲霉菌落。用表征的曲霉联合体制备纳米银。对细胞外合成的纳米颗粒进行了表征。采用MTT法评价银纳米颗粒对MCF7细胞株的抗癌活性。测定IC 50值。结果:曲霉联合体由黑曲霉、米歇尔曲霉和日本曲霉组成。通过硝酸银(agno3)还原成金属银(Ag +)离子,在细胞外合成了纳米银,其颜色由淡黄色变为深红色。紫外-可见分光光度计监测溶液时,在420 nm处观察到恒定的位移。FT-IR光谱证实了- nh3等重要官能团的存在。研究了银纳米颗粒对MCF7的抗癌活性。当AgNPs浓度分别达到25µg、50µg和100µg时,细胞抑制率为100%。值得注意的是,日本刺参纳米颗粒的ic50值最低,为1.47µg/ml。结论:曲霉联合体是生产具有潜在抗癌活性的纳米银的理想真菌生物系统。
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