M. Streďanský, A. Pizzariello, S. Stredanska, S. Miertus
{"title":"Determination of D-fructose in foodstuffs by an improved amperometric biosensor based on a solid binding matrix","authors":"M. Streďanský, A. Pizzariello, S. Stredanska, S. Miertus","doi":"10.1039/A900048H","DOIUrl":null,"url":null,"abstract":"An improved amperometric biosensor based on a solid binding matrix (SBM) composite transducer has been used for the determination of D-fructose in foodstuffs samples. The enzyme, D-fructose dehydrogenase (EC 1.1.99.11), was incorporated directly into a solid composite transducer containing both 2-hexadecanone as SBM and chemically modified graphite. Hexacyanoferrate(III) was used as a redox mediator and the current variation caused by the presence of D-fructose was measured amperometrically. The electrochemical properties and the characteristics of the composite fructose biosensors are described. The amperometric signals were fast, reproducible and linearly proportional to D-fructose concentrations in the range 50 × 10–6–10 × 10–3 mol l–1, with a correlation coefficient of 0.999. A set of measurements at +0.20 V versus SCE for 2 × 10–3 mol l–1D-fructose yielded a relative standard deviation for the steady-state current of 2.11%. The use of a chemically modified graphite by a mild oxidation step was shown to improve the biosensor selectivity against anionic interferents such as L-ascorbate. The biosensor proved to be stable for 6 months and the assay of D-fructose by this electrode was not influenced by the presence of sugars or other interferents commonly found in food samples. The biosensor was used for the determination of D-fructose in some food samples, and the results were consistent with those obtained with the commercially available D-fructose enzyme photometric kit.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"118 1","pages":"57-61"},"PeriodicalIF":0.0000,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"34","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/A900048H","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 34
Abstract
An improved amperometric biosensor based on a solid binding matrix (SBM) composite transducer has been used for the determination of D-fructose in foodstuffs samples. The enzyme, D-fructose dehydrogenase (EC 1.1.99.11), was incorporated directly into a solid composite transducer containing both 2-hexadecanone as SBM and chemically modified graphite. Hexacyanoferrate(III) was used as a redox mediator and the current variation caused by the presence of D-fructose was measured amperometrically. The electrochemical properties and the characteristics of the composite fructose biosensors are described. The amperometric signals were fast, reproducible and linearly proportional to D-fructose concentrations in the range 50 × 10–6–10 × 10–3 mol l–1, with a correlation coefficient of 0.999. A set of measurements at +0.20 V versus SCE for 2 × 10–3 mol l–1D-fructose yielded a relative standard deviation for the steady-state current of 2.11%. The use of a chemically modified graphite by a mild oxidation step was shown to improve the biosensor selectivity against anionic interferents such as L-ascorbate. The biosensor proved to be stable for 6 months and the assay of D-fructose by this electrode was not influenced by the presence of sugars or other interferents commonly found in food samples. The biosensor was used for the determination of D-fructose in some food samples, and the results were consistent with those obtained with the commercially available D-fructose enzyme photometric kit.