Selma Merza Hasan Al-Mousawi, Akram Faisal Alhuwaiz
{"title":"Coating Orthodontic Miniscrew with Chlorhexidine Hexametaphosphate Nanoparticle (An in vitro-study)","authors":"Selma Merza Hasan Al-Mousawi, Akram Faisal Alhuwaiz","doi":"10.36330/kmj.v18i2.3681","DOIUrl":null,"url":null,"abstract":"Introduction: Inflammation associated with insertion of orthodontic miniscrews (OMS) may lead to their failure, therefore inhibition of microbial buildup is preferable. Chlorhexidine hexametaphosphate has offered persistent, slow release of active chlorhexidine over time, and hence a good anti-microbial agent.\nAims of study: To prepare and characterize CHX-HMP antimicrobial nanoparticle for coating and characterization of the stainless steel and titanium orthodontic miniscrews.\nMaterials and Methods: Suspension of 5 mM CHX-HMP nanoparticles were prepared. The formulated suspension was subjected to examination by AFM and FTIR. Sterilized miniscrews were immersed in the prepared suspension for half a minute, then inserted in deionized water for 10 seconds to eliminate any unbound material and then left to dry in air for at least 1 hour. The OMSs were examined by using SEM and FeSEM.\nResults: The AFM micrographs displayed that the characterization of nanoparticles expressed a relative homogeneity in terms of particle size and in their topographical distribution with the regular shaped particles being well aligned vertically with mean particles size 45.2 nm. FTIR showed the presence of C=O bond in chlorhexidine digluconate and its absence in the mixed nanoparticles indicate the replacement of gluconate by hexametaphosphate. SEM and FeSEM showed that all coated OMS (stainless steel and titanium) revealed nanoparticles of spherical shape with perfect homogenous distribution with no agglomeration meaning that the coating of OMS with the prepared colloidal suspension have a good dispersion of nanoparticles.\nConclusion: OMS could be coated with antimicrobial CXH-HMP nanoparticles to assist reducing infection associated with the insertion of OMS.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kufa Journal For Veterinary Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36330/kmj.v18i2.3681","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Inflammation associated with insertion of orthodontic miniscrews (OMS) may lead to their failure, therefore inhibition of microbial buildup is preferable. Chlorhexidine hexametaphosphate has offered persistent, slow release of active chlorhexidine over time, and hence a good anti-microbial agent.
Aims of study: To prepare and characterize CHX-HMP antimicrobial nanoparticle for coating and characterization of the stainless steel and titanium orthodontic miniscrews.
Materials and Methods: Suspension of 5 mM CHX-HMP nanoparticles were prepared. The formulated suspension was subjected to examination by AFM and FTIR. Sterilized miniscrews were immersed in the prepared suspension for half a minute, then inserted in deionized water for 10 seconds to eliminate any unbound material and then left to dry in air for at least 1 hour. The OMSs were examined by using SEM and FeSEM.
Results: The AFM micrographs displayed that the characterization of nanoparticles expressed a relative homogeneity in terms of particle size and in their topographical distribution with the regular shaped particles being well aligned vertically with mean particles size 45.2 nm. FTIR showed the presence of C=O bond in chlorhexidine digluconate and its absence in the mixed nanoparticles indicate the replacement of gluconate by hexametaphosphate. SEM and FeSEM showed that all coated OMS (stainless steel and titanium) revealed nanoparticles of spherical shape with perfect homogenous distribution with no agglomeration meaning that the coating of OMS with the prepared colloidal suspension have a good dispersion of nanoparticles.
Conclusion: OMS could be coated with antimicrobial CXH-HMP nanoparticles to assist reducing infection associated with the insertion of OMS.
简介:正畸微型支架(OMS)插入引起的炎症可能导致其失效,因此抑制微生物积聚是可取的。六偏磷酸氯己定具有持续缓慢释放活性氯己定的特性,因此是一种良好的抗菌剂。研究目的:制备CHX-HMP抗菌纳米颗粒,用于不锈钢和钛正畸微型支架的涂层和表征。材料与方法:制备5 mM CHX-HMP纳米颗粒悬浮液。采用原子力显微镜(AFM)和红外光谱(FTIR)对配制的悬浮液进行检测。将灭菌后的微型微生物浸泡在制备好的悬浮液中半分钟,然后将其插入去离子水中10秒以消除任何未结合的物质,然后将其放置在空气中干燥至少1小时。采用SEM和FeSEM对其进行了表征。结果:AFM显微图显示,纳米颗粒在粒径和地形分布方面表现出相对均匀性,规则形状的颗粒在垂直方向上排列良好,平均粒径为45.2 nm。傅里叶变换红外光谱显示,二光酸氯己定中存在C=O键,而混合纳米颗粒中不存在C=O键,表明葡萄糖酸盐被六偏磷酸取代。SEM和FeSEM结果表明,涂层后的OMS(不锈钢和钛)均呈现球形纳米颗粒,分布均匀,无团聚现象,说明制备的胶体悬浮液涂层具有良好的纳米颗粒分散性。结论:抗菌CXH-HMP纳米颗粒可包被OMS,有助于减少OMS插入引起的感染。