Investigation of the Possible Relation of Apelin, Kynurenine and IL-4/IL-10/IL-12/Tnf-alfa Levels with Clinical and Metabolic Parameters in Obesity

Abstract

Purpose Obesity is a medical condition caused by excess body fat that accumulates at a level that can have a negative impact on health. Altered glucose and lipid metabolism, low-grade chronic inflammation play a role in the pathogenesis of obesity and obesity-related metabolic dysfunction. In our study, we aimed to determine the apelin, kynurenine, IL-4/IL-10/IL-12/TNF-α protein levels in obese individuals and healthy control groups, and to investigate the possible relationship between clinical parameters with the data to be obtained. Materials and Methods The levels of Apelin, Kynurenine, IL-4/IL-10/IL-12/TNF-α in serum/plasma samples were measured with enzyme-linked immunosorbent assay. Absorbance of the samples were measured on a microplate reader spectrophotometrically at a wavelength of 450 nm. Results The levels of Kinurenin, IL-4, and IL-12 in the serum were higher in control group than in obese patients (p=0.009, p=0.004, p=0.002, respectively). There was no significant difference between the control and obese patients in TNF-α, IL-10 and Apelin levels (p=0.277, p=0.711, p=0.472, respectively). Conclusion Inflammation and altered immune response are two important components of obesity. They play a major role in the formation of metabolic diseases associated with obesity. Changes in adipokine levels may lead to the development and maintenance of insulin resistance and systemic inflammation in obesity. The results demonstrate that kynurenine, IL-4, and IL-12 have a complex role in obesity and can be used as therapeutic targets.