Isolation and Identification of Endophytic Fungi from Melia azedarach L. of Kalvarayan Hills, Eastern Ghats, Tamil Nadu, India

S. Kanaga, Dhandapani Ramamurthi, K.C.P ajamanikandan, D. Prasanth
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Abstract

To investigate the Endophytic fungi that are occupied asymptomatically in domestic tissues of all higher plants are of growing interest as promising sources of biologically active agents. The exploitation of novel and eco-friendly secondary metabolites used in medicine, the pharmaceutical industries and agriculture. In this research we select the Melia azedarach L. medicinal plant (exotic tree from Kalvarayan hills) to isolate and identify the endophytic fungi from living apparently symptomless flower, stems, leaves and fruits of medicinal plant will grow very well and is apparently resistant to many pathogenic microorganisms like bacteria, fungi and parasitic infection and it has insecticidal compound. Each segment from the plant of leaves, flowers, fruits and stem each 15 segment of plant parts were be sampled and, total of 47 fungi were isolated. The isolates of endophytic fungi from this plant parts including Cylindrocarpon lichenicola followed by, Chaetomium spp., Curvularia fallax, Fusarium solani, Alternaria alternata, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Aspergillus nidulans. The study the shows the hyphomycetes were more than dematiaceous and mycelia sterilia. The genera of Chaetomium lichenicola and Aspergillus were the most common in that plant.
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印度泰米尔纳德邦Kalvarayan Hills苦楝内生真菌的分离与鉴定
研究在所有高等植物组织中无症状占据的内生真菌作为生物活性物质的有前途的来源越来越受到人们的关注。用于医药、制药工业和农业的新型、生态友好的次生代谢物的开发。本研究选取苦楝(Melia azedarach L.)药用植物(Kalvarayan hills的外来树种),从活的、明显无症状的药用植物花、茎、叶和果实中分离鉴定内生真菌,该植物生长良好,对细菌、真菌和寄生虫感染等多种病原微生物具有明显的抗性,并具有杀虫化合物。从植物的叶、花、果、茎各部分取样,每15个部分,共分离真菌47株。从该植物部位分离到的内生真菌主要有地衣圆柱菌,其次是毛毛菌、黄曲霉、番茄枯萎菌、互交霉、烟曲霉、黑曲霉、黄曲霉和空心曲霉。研究表明,菌丝菌数量多于赤丝菌和无菌菌丝。其中地衣毛菌属和曲霉属最为常见。
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