SELENIUM – GLUTATHIONE PEROXIDASE RELATION IN ERYTHROCYTES WITH G-6-PDH DEFICIENCY AND THE GP ACTIVITY DEVIATION DURING OXIDATION BY SELENIUM. MINI-REVIEW

Abstract

Selenium status and glutathione peroxidase (GP) activity were investigated in patients with normal and glucose-6-phosphate dehydrogenase (G-6-PDH) deficient erythrocytes among Azerbaijani population. It has been shown that the content of Se in G-6-PDH-deficient erythrocytes is a little (≈16%) different from the norm, while GP activity of in them is substantially lower (≈ 50%). The low activity of GP probably is due to decreased production of GSH, the basic substrate for GP, in G-6-PDH-deficient erythrocytes. Addition of GSH precursor (N-acetylcysteine) to the incubation medium increases the GP activity, suggesting that the low level of GP activity is possibly connected with low level of GSH. G-6-PDH-deficient erythrocytes demonstrate significantly greater susceptibility to oxidative stress than normal ones when exposed to ultraviolet radiation of the small (7 kJ/m2) and moderate (15 kJ/m2) doses, and to high tension electric field (HTEF ≈ 60 kV/m × 5 hours). In particular, the accumulation of lipid peroxidation products (malonic dialdehyde) under visible UV irradiation in G-6-PDH-deficient erythrocytes is ≈50-80% higher, than in the control. Nevertheless, in G-6-PDH-deficient erythrocytes HTEF have no significant effect on the accumulation of malonic dialdehyde, though the GP and catalase activity are falling faster than normal, and have initially lower level.