{"title":"Determination of concentration of the pseudomycelles from fermentation broth via spectroscopy and Neubauer chamber","authors":"A. S. Bertan, M. Cremasco","doi":"10.5281/ZENODO.3987017","DOIUrl":null,"url":null,"abstract":"The present work presents an alternative technique to obtain the concentration of pseudomycelles as number of pseudomycelles/sample volume, obtained by Neubauer chamber, as function of absorbance from spectroscopy. Samples of broth from fermentation via bacteria Streptomyces tsukubaensis were analyzed, making it possible to obtain a linear relationship between the number of pseudomycelles/ sample volume and absorbance, with determination coefficient of 0.995. In addition, a morphological analysis of the unfermented pseudomycelles was performed using scanning electron microscopy (SEM) at the magnifications of 20, 2000, 5000, 10000 and 20000 x. \nDOI: http://dx.doi.org/10.5281/zenodo.3987017","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"32 1","pages":"263-270"},"PeriodicalIF":0.0000,"publicationDate":"2020-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Biological Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5281/ZENODO.3987017","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
The present work presents an alternative technique to obtain the concentration of pseudomycelles as number of pseudomycelles/sample volume, obtained by Neubauer chamber, as function of absorbance from spectroscopy. Samples of broth from fermentation via bacteria Streptomyces tsukubaensis were analyzed, making it possible to obtain a linear relationship between the number of pseudomycelles/ sample volume and absorbance, with determination coefficient of 0.995. In addition, a morphological analysis of the unfermented pseudomycelles was performed using scanning electron microscopy (SEM) at the magnifications of 20, 2000, 5000, 10000 and 20000 x.
DOI: http://dx.doi.org/10.5281/zenodo.3987017
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