Recent progresses in plant single-cell transcriptomics

Dihuai Zheng , Jiwei Xu , Yaqian Lu , Hongyu Chen , Qinjie Chu , Longjiang Fan
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Abstract

High-throughput sequencing technologies at single-cell resolution have great potential to reveal a new landscape of plant cells. Single-cell/nucleus RNA (scRNA/snRNA), single-cell/nucleus assay for transposase accessible chromatin (scATAC/snATAC) and spatial transcriptome sequencing have been applied in multiple plant tissues. Consequently, a significant increase in publications on plant single-cell transcriptomics was seen in the recent two years. In this review, we will summarize the advantages and weaknesses of these single-cell sequencing approaches, offer a glimpse of their developments in cell biology, bioinformatic tools and databases in the latest two years.

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植物单细胞转录组学研究进展
单细胞高通量测序技术在揭示植物细胞的新景观方面具有巨大的潜力。单细胞/细胞核RNA (scRNA/snRNA)、转座酶可及染色质单细胞/细胞核测定(scATAC/snATAC)和空间转录组测序已在多种植物组织中得到应用。因此,在最近两年中,植物单细胞转录组学的出版物显著增加。在本文中,我们将总结这些单细胞测序方法的优点和缺点,并简要介绍近两年来它们在细胞生物学、生物信息学工具和数据库方面的发展。
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