Field Qualitative Detection of Avian Influenza Using Antigen Rapid Test in Different Districts of Nineveh Governorate, Iraq

Basila Ibrahim Hussein, Omer Ra’ad Al-Hyalee
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Abstract

Sensitive and rapid diagnostic kits for avian influenza virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. The aim of study is to investigate on avian influenza virus type A serotype H5 among different avian species in different district of Nineveh Governorate, Iraq using Anigen rapid H5 AIV Ag test kit. In this study, we employed One-step avian influenza virus type A and serotype H5 antigen test (Anigen rapid H5 AIV Ag test kit). The test was run out in different districts of Nineveh governorate, including broiler farms local poultry markets, and house poultry breedes (local breeds, broiler, and layers) as well as pigeon houses. Of tested 686 swab from total 241477 poultry samples were been tested, no avian influenza virus positive cases result were noted or confirmed by rapid screening test. No clinical signs were recorded in all examined poultry species. The study conclude that kit used for rapid diagnosis of avian influenza type A serotype H5 was easy, reliable and its interpretation less than thirty minutes.
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利用抗原快速检测法在伊拉克尼尼微省不同地区实地定性检测禽流感
需要灵敏和快速的禽流感病毒诊断试剂盒,以便在疾病暴发期间筛选大量样本并防止感染的传播。采用Anigen快速H5抗体检测试剂盒,对伊拉克尼尼微省不同地区不同禽种间的A型血清H5禽流感病毒进行检测。本研究采用一步法检测甲型禽流感病毒和血清型H5抗原(抗原快速检测试剂盒)。该试验在尼尼微省的不同地区进行,包括肉鸡养殖场、当地家禽市场、家养家禽品种(当地品种、肉鸡和蛋鸡)以及鸽舍。在共241477个家禽样本中检测的686个拭子中,没有发现或通过快速筛选试验确认禽流感病毒阳性病例。所有被检查的家禽品种均未记录到临床症状。本研究认为,用于禽流感A型H5血清型快速诊断的试剂盒操作简单、可靠,诊断时间不超过30分钟。
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