Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies

Balasundari Ramesh, Jaikanth Chandrasekaran, S. Jeevankumar, G. Jacob, K. Cherian
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引用次数: 7

Abstract

Fabrication of 3D novel scaffolds is very challenging and critical to achieve the appropriate function for tissue regeneration. This study aims to develop a hybrid biological nano-scaffold using Liquid nitrogen (LN2) decellularised human amniotic membrane (hAM), human umbilical cord derived collagen nano-fibres and green silver nanoparticles obtained by green synthesis of curcumin. A novel attempt was made to develop in vitro bioengineered dermal layer by seeding the scaffold with cord blood derived Mesenchymal stem cells (CBMSCs) and trans-differentiating them to keratinocytes and dermal fibroblast like cells using keloid foreskin conditioned media. Furthermore, the small animal experiments in Albino Wistar rats prove it to be a safe healing product without scar formation. This novel scaffold can be a promising dressing material for none healing ulcers and burns. This scaffold has long shelf life, easy to apply, have sustained/controlled release of silver, the minimum frequency of dressing changes, manage excessive exudates, moisture retainment, reduce inflammation and facilitate autolytic debridement.
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混合羊膜敷料与绿色纳米银作为生物工程皮肤伤口和烧伤:一个试点研究
3D新型支架的制造是非常具有挑战性的,也是实现组织再生功能的关键。本研究旨在利用液氮(LN2)脱细胞人羊膜(hAM)、人脐带源性胶原纳米纤维和绿色合成姜黄素获得的绿色纳米银,开发一种混合型生物纳米支架。采用脐带血源性间充质干细胞(CBMSCs)植入支架,并利用瘢痕疙瘩包皮条件培养基将其转化为角质形成细胞和真皮成纤维细胞样细胞,构建体外生物工程真皮层。此外,在白化Wistar大鼠身上进行的小动物实验证明,它是一种安全的愈合产品,不会形成疤痕。这种新型支架是治疗无法愈合的溃疡和烧伤的一种很有前途的敷料。这种支架保质期长,易于使用,具有持续/控制释放银,更换敷料的频率最小,管理过多渗出物,保持水分,减少炎症和促进自溶清创。
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