Bactericidal activity of bio mediated silver nanoparticles synthesized by Serratia nematodiphila

Abstract

Objectives

Microbe mediated synthesis of silver nanoparticles by using Serratia nematodiphila and to explore the antibacterial activity against pathogenic bacteria Bacillus subtilis, Klebsiella planticola and Pseudomonas aeruginosa.

Methods

The present studies, 1 mM of silver nitrate was added into 100 ml of S. nematodiphila (CAA) culture supernatant. The bioreduction of pure AgNO₃ were characterized by UV–visible spectroscopy, X-ray diffraction analysis (XRD), transmission electron microscopy (TEM) and Fourier transform infra-red (FTIR) analysis.

Results

In this report, biosynthesized silver nanoparticles are confirmed by color changes and it was characterized by UV–visible spectrum of surface plasmon resonance at 420 nm. Transmission electron microscopy (TEM) showed the formation of well-dispersed silver nanoparticles in the range of 10–31 nm and X-ray diffraction value obtain from range of (200) confirmed synthesized silver nanoparticles in crystalline nature. The microbe mediated synthesized silver nanoparticles shows more zone of inhibition against the pathogenic bacteria B. subtilis, K. planticola and P. aeruginosa.

Conclusion

Biosynthesis of metal nanoparticles is an expanding research area due to the biomedical application for the growth of novel biotechnologies. The report suggests that the synthesized silver nanoparticles act as eco-friendly antibacterial agent.