In vitro Callus induction of Fenugreek (Trigonellafoenum-graecum L.) Using Different Media with Different Auxins Concentrations

Abstract

A protocol of callus induction in fenugreek (Trigonellafoenum-graecumL.) from 8 20 days old in vitro grown cotyledons node explants cultured on Murashige and Skoog (MS) and B5((Gamborget al., 1958) media supplemented with different types and concentrations of growth regulators were tested in order to obtain the best callus formation. Two auxin types, αnaphthalene acetic acid (NAA), and 2,4-dichloro-phenoxyacetic acid (2,4-D) at four concentrations(0.5, 0.1, 1.5, and 2.0 mgL-1) with (0,0) as control in the two media were used in this study .The objective of this study was to determine the effect of different concentration of two types of auxins 2,4-D (2,4Dichlorophenoxy acetic acid) and NAA (Naphthalene acetic acid), on callus induction using cotyledons and hypocotyls explants of T.foenum –graecum. 100 percentage of primary callus induction rate and 2.5 callusing index were achieved on MS medium containing 2mg/l ,2,4-D hormone hypocotyls explants .The maximum value of callusing index (2.8) was obtained from MS medium containing 1.5 mg/l ,2,4D using hypocotyls and cotyledons explants. The maximum callus formation observed in the MS media containing 2.0mg/l NAA was 3.9±o.o8 in hypocotyls segment. The callus was compact in cotyledons and variable in hypocotyls segments and the colour is creamy.