METABOLIC CHANGES IN THE ORGANISM OF SHEEP AT THE LAST TERMS OF GESTATION WITH COMPLICATION OF PREGNANCY WITH ECLAMPSIAS

Abstract

During the prophylactic examination at the last stages of gestation, 27.691.79% of pregnant ewes were diagnosed with eclampsia of varying severity: arterial hypertension (BP 136.1 ± 2.85 mm Hg), proteinuria (protein content in urine more than 3 0±0.49 g/l), edema and coma. In urine, an increase in the concentration of ketone bodies above the physiological norm by 2.3 times, their fractions of AcAc and BH - by 5.9 and 1.5 times, respectively, was noted. In the blood, a decrease in buffer bases to 18.41 ± 1.53 mmol/l, a glucose concentration to 2.12 ± 0.12 mmol/l, a BH/AcAc coefficient to 1.47 ± 0.12 mmol/l were established. There was an increase in the concentration of the intermediate product of lipid peroxidation in the blood by 43.0%, the concentration of stable nitric oxide metabolites - by 38.0%, vitamin C - by 24.1%. The content of vitamin E decreased by 13.1%, and the concentration of double bonds increased by 20.46% with an atypical form of eclampsia and by 34.13% with the appearance of a typical form of eclampsia. The level of diene conjugates increased by 1.87 times. The concentration of intermediate products of ketodienes and conjugated trienes is increased by 1.75 times in comparison with the atypical form of the course of eclampsia and by 3.54 times with its typical course. In pregnant ewes, the activity of oxidized glutathione is increased (2.879 ± 0.32 µmol/l), and superoxide dismutase (1.736±0.37 arb. units) is lower than in the comparison group (2.146±0.56 µmol/l). Consequently, the metabolic parameters that are traditionally used in the diagnostic algorithm in pregnant sheep with metabolic stress with symptoms of eclampsia in some cases are less sensitive and specific than the indicators of the system "lipid peroxidation - antioxidant protection". In the future, the material obtained in this work should be taken into account when studying the problem of eclampsia in pregnant sheep, as a concept for the development of impaired functioning of the lipid peroxidation-antioxidant protection system in this metabolic pathology.