Total phenolic, flavonoid contents and antioxidant activity of tamarind seed and pulp extracts

Le Phuong Ha, Nguyen Van Ngoc, Nguyen Thi Trang Huyen, Le Thi Thu Hang, Nguyen Thi Kieu Oanh, Tran Mai Thi Tuyet, Nguyen Thi Mai Phuong, N. M. Minh Hong
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引用次数: 1

Abstract

Tamarind (Tamarindus indica) has long been known for its high nutrition content and pharmacological potential. However, there is lack of studies on the content of antioxidants, phenolic and flavonoid contents of tamarind seed grown in Vietnam. Thus, the aim of this study was to compare the seeds and pulps of Tamarindus indica from three different areas across Vietnam including Son La, Hai Phong and Sai Gon with regard to the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of their water and methanol extracts, as well as their cytotoxicity on a normal BKH-21cells. TPC and TFC were evaluated by the Folin–Ciocalteu reagent and aluminum chloride, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays were used to investigate antioxidant capacity. The safety of T. indica extracts was assessed by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Our results showed that the methanolic extracts yielded higher TPC (742.919 ± 50.360 mg GAE/g extract), TFC (68.492 ± 0.023 mg QE/g extract) and possessed stronger free radical scavenging activity (IC50 of 52.5 µg/mL) compared to that of water extracts. T. indica seeds from all three regions possessed higher TPC, TFC and antioxidant activity than those of pulps. Regarding the safety, in vitro analysis showed that tamarind seed and pulp extracts only became toxic to BHK-21 cell line at a very high concentration with IC50 values range from 143.77 µg/mL to 620.35 µg/mL. This study revealed that T. indica seeds and pulps can serve as functional food as well as potential antioxidants in pharmaceutical products.
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罗望子籽和果肉提取物的总酚、类黄酮含量及抗氧化活性
罗望子(Tamarindus indica)长期以来以其高营养含量和药理潜力而闻名。然而,对越南产罗望子中抗氧化剂、酚类和类黄酮含量的研究较少。因此,本研究的目的是比较越南三个不同地区(山腊、海防和西贡)的柽柳种子和果肉的总酚含量(TPC)、总黄酮含量(TFC)、水提取物和甲醇提取物的抗氧化活性,以及它们对正常bhk -21细胞的细胞毒性。分别用Folin-Ciocalteu试剂和氯化铝评价TPC和TFC。采用2,2-二苯基-1-吡啶肼(DPPH)和2,2'-氮化萘(3-乙基苯并噻唑-6-磺酸(ABTS))自由基清除实验研究抗氧化能力。采用MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑)测定法评价籼稻提取物的安全性。结果表明,与水提取物相比,甲醇提取物的TPC(742.919±50.360 mg GAE/g提取物)和TFC(68.492±0.023 mg QE/g提取物)具有更高的自由基清除活性(IC50为52.5µg/mL)。三个地区的籼稻种子的TPC、TFC和抗氧化活性均高于纸浆。体外安全性分析表明,罗望子籽和果肉提取物仅在非常高浓度时才对BHK-21细胞株产生毒性,IC50值为143.77 ~ 620.35µg/mL。本研究表明,籼稻种子和果肉既可作为功能性食品,又可作为潜在的抗氧化剂用于医药产品。
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