Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19532
Ly Khanh Linh, Nguyen Van Doai, Nguyen Thi Linh, P. Quyen, Chu Hoang Ha, Pham Bich Ngoc, Do Tien Phat
Rice is one of the most important staple food crops worldwide, especially in Asia. However, the climate change has been negatively affecting the arable land area, which leads to a severe reduction of the rice yield. In which, the salt stress is considered as one of the most critical abiotic stresses. The creation of rice varieties with salt tolerance is an urgent research direction. In this study, we successfully created two CRISPR/Cas9 vectors to introduce targeted mutations of two different positions in the promoter region of the OsSRFP1 , a gene involves in the Ubiquitination pathway and regulates important responses to multiple stresses. Cali from mature seeds of the Khang Dan 18 rice cultivar was used for Agrobacterium mediated transformation. Total 6 T0 rice lines showed CRISPR/Cas9 induced mutations in the targeted sites. Two of them were utilized for validating inheritance and segregation of induced mutations at the T1 generation. Importantly, homozygous mutant lines lacking the transgene were successfully identified at the T1 generation. These mutant lines are valuable materials for us to continue to assess gene expression, plant growth and development as well as salt tolerance in the further research work.
{"title":"Development of CRISPR/Cas9 systems to induce targeted mutations in the promoter region of the OsSRFP1 gene in rice","authors":"Ly Khanh Linh, Nguyen Van Doai, Nguyen Thi Linh, P. Quyen, Chu Hoang Ha, Pham Bich Ngoc, Do Tien Phat","doi":"10.15625/1811-4989/19532","DOIUrl":"https://doi.org/10.15625/1811-4989/19532","url":null,"abstract":"Rice is one of the most important staple food crops worldwide, especially in Asia. However, the climate change has been negatively affecting the arable land area, which leads to a severe reduction of the rice yield. In which, the salt stress is considered as one of the most critical abiotic stresses. The creation of rice varieties with salt tolerance is an urgent research direction. In this study, we successfully created two CRISPR/Cas9 vectors to introduce targeted mutations of two different positions in the promoter region of the OsSRFP1 , a gene involves in the Ubiquitination pathway and regulates important responses to multiple stresses. Cali from mature seeds of the Khang Dan 18 rice cultivar was used for Agrobacterium mediated transformation. Total 6 T0 rice lines showed CRISPR/Cas9 induced mutations in the targeted sites. Two of them were utilized for validating inheritance and segregation of induced mutations at the T1 generation. Importantly, homozygous mutant lines lacking the transgene were successfully identified at the T1 generation. These mutant lines are valuable materials for us to continue to assess gene expression, plant growth and development as well as salt tolerance in the further research work.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"30 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19591
N. T. Tra, Ngo Hong Duong, Trinh Thai Vy, Le Thi Tra My, Nguyen Thi Thu Hien, Pham Thi Van, Doan Thi Thanh Huong, Pham Bich Ngoc, Chu Hoang Ha, Hoang Thi Thu Hang
Infectious bronchitis virus (IBV) is considered as one of the main causes of economic loss in chicken farms worldwide, especially in poultry producing nations. This virus, a member of the Coronaviridae family, is classified into different genotypes based on its surface spike glycoproteins which also play important roles in cell attachment and immune response. Due to continual genetic mutation of IBV, attenuated and inactivated vaccines show decreased effectiveness or lack of cross – protection; thus ongoing studies focus on the development of new generation vaccines to prevent new IBV outbreaks. This paper describes our study on expression of the antigenic region in S1 subunit of the spike in Nicotiana benthamiana. Two expression vectors carrying either S1 or receptor binding domain (RBD) coding gene (pCB301-S1 and pCB301-RBD) were constructed and transformed into tobacco leaves by agroinfiltration method. The RBD showed a clearly higher level of expression compared to the whole S1. Purification of RBD by immobilized metal ion chromatography and size exclusion chromatography represented a mixture of monomer, dimer and trimer glycoprotein with expected sizes in Western blot. In summary, this study demonstrated our primary success in establishing an expression model that could be used to investigate plant-based IBV recombinant vaccine.
{"title":"Study on the transient expression of infectious bronchitis virus spike protein in Nicotiana benthamiana leaves","authors":"N. T. Tra, Ngo Hong Duong, Trinh Thai Vy, Le Thi Tra My, Nguyen Thi Thu Hien, Pham Thi Van, Doan Thi Thanh Huong, Pham Bich Ngoc, Chu Hoang Ha, Hoang Thi Thu Hang","doi":"10.15625/1811-4989/19591","DOIUrl":"https://doi.org/10.15625/1811-4989/19591","url":null,"abstract":"Infectious bronchitis virus (IBV) is considered as one of the main causes of economic loss in chicken farms worldwide, especially in poultry producing nations. This virus, a member of the Coronaviridae family, is classified into different genotypes based on its surface spike glycoproteins which also play important roles in cell attachment and immune response. Due to continual genetic mutation of IBV, attenuated and inactivated vaccines show decreased effectiveness or lack of cross – protection; thus ongoing studies focus on the development of new generation vaccines to prevent new IBV outbreaks. This paper describes our study on expression of the antigenic region in S1 subunit of the spike in Nicotiana benthamiana. Two expression vectors carrying either S1 or receptor binding domain (RBD) coding gene (pCB301-S1 and pCB301-RBD) were constructed and transformed into tobacco leaves by agroinfiltration method. The RBD showed a clearly higher level of expression compared to the whole S1. Purification of RBD by immobilized metal ion chromatography and size exclusion chromatography represented a mixture of monomer, dimer and trimer glycoprotein with expected sizes in Western blot. In summary, this study demonstrated our primary success in establishing an expression model that could be used to investigate plant-based IBV recombinant vaccine.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"40 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140728158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/17315
Ho Thi Bich Phuong, Nguyen Dang Quan, Phan Thi Kim Tram, Tang Ha Nam Anh, Le Thi Truc Linh
MicroRNA144-3p was reported to associate with osteoarthritis (OA) since it was upregulated in this disease. ABCA1 was also found to involve in OA. Bioinformatics algorithms showed ABCA1 was potential targets of miR-144-3p. This study aims to prove ABCA1 is direct targets of miR-144-3p experimentally. Expression of ABCA1 was determined by Realtime RT-PCR after performing the gain- and loss- function of miR-144-3p in chondrocyte. The 3’UTR containing several binding sites of miR-144-3p was subcloning. The vector with binding sites of miR-144-3p mutated was aslo created. Luciferase assay was performed to check the ability of miR-144-3p binding to ABCA1. Realtime RT-PCR showed that the overexpression of miR-144-3p inhibited ABCA1 expression. The Luciferase assay showed that miR-144-3p directly interacted with ABCA1 through its binding sites on the 3’UTR. These data suggested that ABCA1 is the direct target of miR-144-3p.
{"title":"ABCA1 is direct target gene of miR-144-3p in chondrocyte","authors":"Ho Thi Bich Phuong, Nguyen Dang Quan, Phan Thi Kim Tram, Tang Ha Nam Anh, Le Thi Truc Linh","doi":"10.15625/1811-4989/17315","DOIUrl":"https://doi.org/10.15625/1811-4989/17315","url":null,"abstract":"MicroRNA144-3p was reported to associate with osteoarthritis (OA) since it was upregulated in this disease. ABCA1 was also found to involve in OA. Bioinformatics algorithms showed ABCA1 was potential targets of miR-144-3p. This study aims to prove ABCA1 is direct targets of miR-144-3p experimentally. Expression of ABCA1 was determined by Realtime RT-PCR after performing the gain- and loss- function of miR-144-3p in chondrocyte. The 3’UTR containing several binding sites of miR-144-3p was subcloning. The vector with binding sites of miR-144-3p mutated was aslo created. Luciferase assay was performed to check the ability of miR-144-3p binding to ABCA1. Realtime RT-PCR showed that the overexpression of miR-144-3p inhibited ABCA1 expression. The Luciferase assay showed that miR-144-3p directly interacted with ABCA1 through its binding sites on the 3’UTR. These data suggested that ABCA1 is the direct target of miR-144-3p.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"98 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140731752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19530
Nguyen Thanh Thuy, Pham Tien Duc, Tran Huu Dinh, Nguyen Thuy Duong
Infertility is a complex disease that is characterized by the failure to achieve pregnancy after 24 months of regular, unprotected sexual intercourse. Various factors result in male sterility, including genetic and non-genetic factors. Recently, scientists have drawn attention to the role of metabolic genes in contributing to the risk of male infertility by inducing reactive oxygen species (ROS). However, such studies about N-acetyltransferase 2 (NAT2), an enzyme that participates in phase II metabolism, remain limited. Thus, this study investigated whether the NAT2 rs1799931 variant was associated with idiopathic male sterility in Vietnam. A total of 306 DNA samples (148 cases and 158 controls) were genotyped using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. About 20% of randomly selected samples were confirmed using an allele-specific polymerase chain reaction (AS-PCR). The results showed that genotype frequencies were consistent with Hardy-Weinberg Equilibrium. Notably, there was an association between NAT2 rs1799931 and male infertility in two genetic models: the additive model (GA genotype, OR = 0.606, 95% CI: 0.369–0.989, p-value = 0.044) and the dominant model (GA+AA genotypes, OR = 0.614, 95% CI: 0.382–0.981, p-value = 0.040). This study has enriched our knowledge about the roles of genetic factors in contributing to male infertility in the Vietnamese population.
{"title":"Association study of NAT2 rs1799931 polymorphism with male infertility","authors":"Nguyen Thanh Thuy, Pham Tien Duc, Tran Huu Dinh, Nguyen Thuy Duong","doi":"10.15625/1811-4989/19530","DOIUrl":"https://doi.org/10.15625/1811-4989/19530","url":null,"abstract":"Infertility is a complex disease that is characterized by the failure to achieve pregnancy after 24 months of regular, unprotected sexual intercourse. Various factors result in male sterility, including genetic and non-genetic factors. Recently, scientists have drawn attention to the role of metabolic genes in contributing to the risk of male infertility by inducing reactive oxygen species (ROS). However, such studies about N-acetyltransferase 2 (NAT2), an enzyme that participates in phase II metabolism, remain limited. Thus, this study investigated whether the NAT2 rs1799931 variant was associated with idiopathic male sterility in Vietnam. A total of 306 DNA samples (148 cases and 158 controls) were genotyped using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. About 20% of randomly selected samples were confirmed using an allele-specific polymerase chain reaction (AS-PCR). The results showed that genotype frequencies were consistent with Hardy-Weinberg Equilibrium. Notably, there was an association between NAT2 rs1799931 and male infertility in two genetic models: the additive model (GA genotype, OR = 0.606, 95% CI: 0.369–0.989, p-value = 0.044) and the dominant model (GA+AA genotypes, OR = 0.614, 95% CI: 0.382–0.981, p-value = 0.040). This study has enriched our knowledge about the roles of genetic factors in contributing to male infertility in the Vietnamese population.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"85 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19755
Pham Bao Tran, Nguyen Anh Thu, Tong Thi Hang
This study explores the antibiotic susceptibility of Campylobacter, a prominent foodborne pathogen, isolated in Ho Chi Minh city markets and the efficacy of commercial probiotics in inhibiting these bacteria for enhancing food safety and treating Campylobacter infections. Bacteria were isolated from chicken meat in modified coal deoxycholate cefoperazone agar (mCCD), followed by characterization as per standard procedures. Ten isolates with Gram negative, catalase positive and oxidase positive characteristics were collected. Antibiotic susceptibility is ascertained through the determination of the inhibited zone and minimum inhibitory concentration (MIC) of five distinct antibiotics against Campylobacter on Muller Hinton agar plates, culminating in a comprehensive assessment after a 24-hour incubation duration. The antibiotic susceptibility results underscore substantial diversity in Campylobacter isolates among meat samples, thereby accentuating discernible distinctions among the various antibiotic products. The research also evaluated the suitability of 5 commercial probiotic products (re-named as A, B, C, D, and E for fair assessment) by examining their impact on the growth of Campylobacter colonies. The antimicrobial effect of probiotics against Campylobacter is assessed using the agar well diffusion assay and co-culture method. We obtained consistent results from two methods, indicating no variation in Campylobacter species among meat samples but significant variations among probiotic products. The outcomes of this research provide valuable insights into the antimicrobial potential of each probiotic and antibiotic on Campylobacter, informing recommendations for food hygiene practices and underscoring the role of both probiotics and antibiotics in combating Campylobacter infections.
{"title":"Effect of commercial probiotics and antibiotics on the growth of Campylobacter isolated from chicken meat in Ho Chi Minh city markets","authors":"Pham Bao Tran, Nguyen Anh Thu, Tong Thi Hang","doi":"10.15625/1811-4989/19755","DOIUrl":"https://doi.org/10.15625/1811-4989/19755","url":null,"abstract":"This study explores the antibiotic susceptibility of Campylobacter, a prominent foodborne pathogen, isolated in Ho Chi Minh city markets and the efficacy of commercial probiotics in inhibiting these bacteria for enhancing food safety and treating Campylobacter infections. Bacteria were isolated from chicken meat in modified coal deoxycholate cefoperazone agar (mCCD), followed by characterization as per standard procedures. Ten isolates with Gram negative, catalase positive and oxidase positive characteristics were collected. Antibiotic susceptibility is ascertained through the determination of the inhibited zone and minimum inhibitory concentration (MIC) of five distinct antibiotics against Campylobacter on Muller Hinton agar plates, culminating in a comprehensive assessment after a 24-hour incubation duration. The antibiotic susceptibility results underscore substantial diversity in Campylobacter isolates among meat samples, thereby accentuating discernible distinctions among the various antibiotic products. The research also evaluated the suitability of 5 commercial probiotic products (re-named as A, B, C, D, and E for fair assessment) by examining their impact on the growth of Campylobacter colonies. The antimicrobial effect of probiotics against Campylobacter is assessed using the agar well diffusion assay and co-culture method. We obtained consistent results from two methods, indicating no variation in Campylobacter species among meat samples but significant variations among probiotic products. The outcomes of this research provide valuable insights into the antimicrobial potential of each probiotic and antibiotic on Campylobacter, informing recommendations for food hygiene practices and underscoring the role of both probiotics and antibiotics in combating Campylobacter infections.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"40 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140728156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19504
Nguyen Huu Hong Thu, Nong Thuy Linh, Ma Thi Huyen Thuong, Nguyen Thuy Duong, Nguyen Hai Ha
An imbalance of glucose and severely high triglyceride are the key characteristics driving hypertriglyceridemia and hyperlipidemic pancreatitis. Hereditary factors considerably promote the increase of triglyceride levels and secondary complications. This study explored the clinical characteristics and genetic causes of a young girl with recurrent severe hypertriglyceridemia-developed pancreatitis at 9-year-olds and diabetes mellitus appearance at 14-year-olds. At 20 years old, she was admitted to the hospital in critical condition and had pancreatitis pain with triglyceride level at 26.22 mmol/L, HbA1c 15%, and glucose at 21.3 mmol/L. Whole exome sequencing analysis showed that she had two heterozygous variants of the APOA5 gene, (p.G185C and p.S19W), one homozygous variant of the APOE gene (p.C156R), which were previously reported to cause critical hypertriglyceridemia condition of patients. The results and analysis propose these variations were the underlying causes of a heightened of patient's plasma triglycerides and lead metabolic complication onset at a young age. These findings shed light on the molecular mechanism of her elevated plasma triglyceride and the early onset of diabetes following hyperlipidemic pancreatitis.
{"title":"Variants APOA5 p.G185C and p.S19W associated with early-onset severe hypertriglyceridemia-induced pancreatitis and diabetes complications in a Vietnamese girl","authors":"Nguyen Huu Hong Thu, Nong Thuy Linh, Ma Thi Huyen Thuong, Nguyen Thuy Duong, Nguyen Hai Ha","doi":"10.15625/1811-4989/19504","DOIUrl":"https://doi.org/10.15625/1811-4989/19504","url":null,"abstract":"An imbalance of glucose and severely high triglyceride are the key characteristics driving hypertriglyceridemia and hyperlipidemic pancreatitis. Hereditary factors considerably promote the increase of triglyceride levels and secondary complications. This study explored the clinical characteristics and genetic causes of a young girl with recurrent severe hypertriglyceridemia-developed pancreatitis at 9-year-olds and diabetes mellitus appearance at 14-year-olds. At 20 years old, she was admitted to the hospital in critical condition and had pancreatitis pain with triglyceride level at 26.22 mmol/L, HbA1c 15%, and glucose at 21.3 mmol/L. Whole exome sequencing analysis showed that she had two heterozygous variants of the APOA5 gene, (p.G185C and p.S19W), one homozygous variant of the APOE gene (p.C156R), which were previously reported to cause critical hypertriglyceridemia condition of patients. The results and analysis propose these variations were the underlying causes of a heightened of patient's plasma triglycerides and lead metabolic complication onset at a young age. These findings shed light on the molecular mechanism of her elevated plasma triglyceride and the early onset of diabetes following hyperlipidemic pancreatitis.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"106 1‐2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140731615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19494
Le Tho Son, Nguyen Thi Hong Gam, Nguyen Thi Thu, Do Thi Hong Loan
Caenorhabditis sinica is a male/female species, in which the genetic diversity is possibly high. Thus, the species is advantageous for the understanding of the mechanisms of diversity, evolution, and adaptation within the nematode genus Caenorhabditis. Previous studies reported the geographic distribution of C. sinica only in China; nonetheless, this should be more convincing for its ecology. We allegedly surveyed the Caenorhabditis species and their diversity in the forests of Vietnam. We found 59 C. sinica strains, and this therefore indicates the vast variation that is 85.88% and 100% identical within isolated wild-type strains. They unequally present in habitats and are enormous in the northern forest, and rarer in the southern forest. The comparison of 18S rDNA barcode sequences from 59 C. sinica isolates by nucleotide sequence alignment showed the consistent diversity among the strains in or off the same ecologies, and all are comparable with the ever-first isolated strain JU727.
{"title":"Wild-type Caenorhabditis sinica, a model nematode for speciation and evolution, massively found in Vietnam","authors":"Le Tho Son, Nguyen Thi Hong Gam, Nguyen Thi Thu, Do Thi Hong Loan","doi":"10.15625/1811-4989/19494","DOIUrl":"https://doi.org/10.15625/1811-4989/19494","url":null,"abstract":"Caenorhabditis sinica is a male/female species, in which the genetic diversity is possibly high. Thus, the species is advantageous for the understanding of the mechanisms of diversity, evolution, and adaptation within the nematode genus Caenorhabditis. Previous studies reported the geographic distribution of C. sinica only in China; nonetheless, this should be more convincing for its ecology. We allegedly surveyed the Caenorhabditis species and their diversity in the forests of Vietnam. We found 59 C. sinica strains, and this therefore indicates the vast variation that is 85.88% and 100% identical within isolated wild-type strains. They unequally present in habitats and are enormous in the northern forest, and rarer in the southern forest. The comparison of 18S rDNA barcode sequences from 59 C. sinica isolates by nucleotide sequence alignment showed the consistent diversity among the strains in or off the same ecologies, and all are comparable with the ever-first isolated strain JU727.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"59 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/19383
Vu Thi Hong Nhung, La Duc Duy, Le Thi Thanh Huong, Nguyen Thuy Duong
Hypohidrotic ectodermal dysplasia (HED) is a rare disease characterized by abnormal development of the structures derived from the ectoderm layer, including hair, teeth, nails, and sweat glands. The most observed form of HED, which is known as X-linked hypohidrotic ectodermal dysplasia (XLHED), is commonly attributed to genetic abnormalities in the ectodysplasin A (EDA) gene. We have successfully discovered a known mutation c.1045G>A (p.A349T) in a Vietnamese proband who displayed the main symptoms of XLHED using PCR and Sanger sequencing. Furthermore, the segregation of the mutation showed that the mother, who exhibited a normal phenotype, was a carrier of the mutation, while the father was hemizygous for the wild-type allele. The identification of the mutation c.1045G>A (p.A349T) contributes to HED research worldwide and can be used for genetic counseling in Vietnam.
{"title":"Detection of an EDA mutation causing hypohidrotic ectodermal dysplasia in a Vietnamese patient","authors":"Vu Thi Hong Nhung, La Duc Duy, Le Thi Thanh Huong, Nguyen Thuy Duong","doi":"10.15625/1811-4989/19383","DOIUrl":"https://doi.org/10.15625/1811-4989/19383","url":null,"abstract":"Hypohidrotic ectodermal dysplasia (HED) is a rare disease characterized by abnormal development of the structures derived from the ectoderm layer, including hair, teeth, nails, and sweat glands. The most observed form of HED, which is known as X-linked hypohidrotic ectodermal dysplasia (XLHED), is commonly attributed to genetic abnormalities in the ectodysplasin A (EDA) gene. We have successfully discovered a known mutation c.1045G>A (p.A349T) in a Vietnamese proband who displayed the main symptoms of XLHED using PCR and Sanger sequencing. Furthermore, the segregation of the mutation showed that the mother, who exhibited a normal phenotype, was a carrier of the mutation, while the father was hemizygous for the wild-type allele. The identification of the mutation c.1045G>A (p.A349T) contributes to HED research worldwide and can be used for genetic counseling in Vietnam.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"125 S179","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140731552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-08DOI: 10.15625/1811-4989/20291
Nguyen Thi Ha Trang, Tran Thu Trang, Nguyen Trung Nam
Poly (I:C) (Polyinosinic:polycytidylic acid) is a synthetic analog of double-stranded RNA binding toll-like receptor 3 (TLR3) and mimicking a virus infection in macrophage. Here, for the first time, we showed the inhibitory effects of eurycomanone on the production of IL-6, TNF-alpha and IL-10 of RAW 264.7 cells stimulated with poly (I:C). Eurycomanone inhibited production of pro-inflammatory cytokines including IL-6 and TNF-alpha of RAW 264.7 cells stimulated with poly (I:C) with the IC50 value being 5.14 +/- 0.60 uM and 2.32 +/- 0.40 uM, respectively. Interestingly, this compound also inhibited the production of anti-inflammatory cytokine IL-10 of RAW 264.7 cells stimulated with poly (I:C) with the IC50 value being 14.60 +/- 0.32 uM. These results suggested that eurycomanone has a potentially regulatory role on production of both pro-inflammatory and anti-inflammatory cytokines in macrophage in the mimicking context of virus infection. Whether eurycomanone inhibits the binding of poly (I:C)-TLR3 or intracellular signaling pathways needs further investigation.
{"title":"Cytokine inhibitory activity of eurycomanone in RAW 264.7 cells stimulated with viral-mimicking poly (I:C)","authors":"Nguyen Thi Ha Trang, Tran Thu Trang, Nguyen Trung Nam","doi":"10.15625/1811-4989/20291","DOIUrl":"https://doi.org/10.15625/1811-4989/20291","url":null,"abstract":"Poly (I:C) (Polyinosinic:polycytidylic acid) is a synthetic analog of double-stranded RNA binding toll-like receptor 3 (TLR3) and mimicking a virus infection in macrophage. Here, for the first time, we showed the inhibitory effects of eurycomanone on the production of IL-6, TNF-alpha and IL-10 of RAW 264.7 cells stimulated with poly (I:C). Eurycomanone inhibited production of pro-inflammatory cytokines including IL-6 and TNF-alpha of RAW 264.7 cells stimulated with poly (I:C) with the IC50 value being 5.14 +/- 0.60 uM and 2.32 +/- 0.40 uM, respectively. Interestingly, this compound also inhibited the production of anti-inflammatory cytokine IL-10 of RAW 264.7 cells stimulated with poly (I:C) with the IC50 value being 14.60 +/- 0.32 uM. These results suggested that eurycomanone has a potentially regulatory role on production of both pro-inflammatory and anti-inflammatory cytokines in macrophage in the mimicking context of virus infection. Whether eurycomanone inhibits the binding of poly (I:C)-TLR3 or intracellular signaling pathways needs further investigation.","PeriodicalId":23622,"journal":{"name":"Vietnam Journal of Biotechnology","volume":"50 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140731990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-26DOI: 10.15625/1811-4989/18086
Nguyen Thi Sam, Nguyễn Thị Huyền, Nguyen T. Phuong‐Anh, Pham The Dan, Nguyen Thi Thuy-Ngan, Nguyen Manh Cuong, Chu Hoang Ha, `Nguyen Trung Nam
Mesenchymal stem cells (MSCs) have become an effective tool for treating immune-related diseases due to their multilineage potential and immunomodulatory capabilities. One of the main factors contributing to their immunomodulatory capabilities is the IDO cascade, which was chosen as the main subject in this research. The IDO-Kyn-AHR-CYP cascade was chosen to evaluate the immunomodulatory properties of treated MSCs, with expression levels of the key gene IDO (indoleamine 2,3-dioxygenase) selected as the screening criterion. Cultured human umbilical cord MSCs (hUC-MSCs) were treated with different natural bioactive compounds. Preliminary results indicated that Rubiadin-3-methyl ether, an anthraquinone derivative isolated from the roots of Morinda longissima, was able to significantly induce IDO2 expression in UC-MSCs but not CYPs. This is the first study to show a link between Rubiadin-3-methyl ether and IDO2 in hUC-MSC. More research is needed to determine whether human UC-MSCs primed with Rubiadin-3-methyl ether have any significant benefits for treating immune-related diseases and disorders.
间充质干细胞(MSCs)由于其多谱系潜能和免疫调节能力已成为治疗免疫相关疾病的有效工具。IDO级联是影响其免疫调节能力的主要因素之一，本研究选择IDO级联作为主要研究对象。选择IDO- kyn - ahr - cyp级联评价处理后MSCs的免疫调节特性，选择关键基因IDO(吲哚胺2,3-双加氧酶)的表达水平作为筛选标准。用不同的天然生物活性化合物处理培养的人脐带间充质干细胞(hUC-MSCs)。初步结果表明，从朗戟根中提取的蒽醌衍生物rubiadin -3-甲基醚能显著诱导UC-MSCs中IDO2的表达，但不能诱导CYPs的表达。这是首次在hUC-MSC中显示rubiadin -3-甲基醚和IDO2之间的联系。需要更多的研究来确定用rubiadin -3-甲基醚引发的人UC-MSCs是否对治疗免疫相关疾病和失调有任何显著的益处。
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