Different methods and carriers for immobilization cellulase from Trichoderma viride and its remaining activity

Abstract

Objective: Many methods for enzymes immobilization and large number of carriers for immobilized enzymes, there are many benefits and diffeculties for immobilization, so the objective of this paper is to determine the suitable method and carrier for cellulase immobilization. Methods: Three methods used for immobilization which they adsorption, Glutaraldehyde and modified glutaraldehyde using two different carriers the first anion exchanger AM-21-A, and the second cation exchange fiber VION KN -1. Results: The result shows that the degree of saving catalytic activity for immobilized and free cellulase were 35, 55, and 75% for the three methods respectively and The result of degree maintain of specific activity for native enzyme were 57%, 42%, for the two carriers VION KN-1, AM-21-A respectively. Conclusions: It can be concluded that the modified glutaraldehyde is the best method for cellulase immobilization and VION KN-1 is better carrier than AM-21-A.