Laccase production and dye decolorization by Trametes versicolor: application of Taguchi and Box-Behnken Methodologies

Abstract

Objective: The aim of this study was to investigate the laccase production of Trametes versicolor under submerged fermentation condition. Then, dye decolorization by laccase was optimized using Box-Behnken methodology. Methods: The optimal culture conditions for producing high amount of laccase were determined using Taguchi methodology. The experiments were designed with five factors (glucose, yeast extract, CuSO 4 , inoculum size and pH) at three levels with orthogonal array layout of L27 (3 5 ). Then, the optimum conditions for high decolorization activity of Reactive Blue 49 by obtained crude laccase were also investigated using Box-Behnken methodology. Results: The optimum culture conditions for production of high amounts of laccase were detected as 2 g L -1 of glucose, 5 g L -1