Studies on Laccase Activity in the Filamentous Fungus Trichoderma reesei

Semra Sekme, N. Ataci, Inci Arisan
{"title":"Studies on Laccase Activity in the Filamentous Fungus Trichoderma reesei","authors":"Semra Sekme, N. Ataci, Inci Arisan","doi":"10.18478/IUFSJB.50042","DOIUrl":null,"url":null,"abstract":"Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IUFS Journal of Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18478/IUFSJB.50042","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
丝状真菌里氏木霉漆酶活性的研究
漆酶的研究主要集中在担子菌科的木腐真菌中,特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明,青苔漆酶在酸性pH范围内具有活性,最适pH为4.5。在含10 gL -1儿茶酚的培养基中,漆酶的活性为1.22 U ml -1,比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下,漆酶活性略有下降,最大活性为1.1 Uml -1。结果表明,在以儿茶酚为碳源的培养基中,灰杉漆酶活性高于葡萄糖。漆酶的研究主要集中在担子菌科的木腐真菌中,特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明,青苔漆酶在酸性pH范围内具有活性,最适pH为4.5。在含10 gL -1儿茶酚的培养基中,漆酶的活性为1.22 U ml -1,比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下,漆酶活性略有下降,最大活性为1.1 Uml -1。结果表明,在以儿茶酚为碳源的培养基中,灰杉漆酶活性高于葡萄糖。漆酶的研究主要集中在担子菌科的木腐真菌中,特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明,青苔漆酶在酸性pH范围内具有活性,最适pH为4.5。在含10 gL -1儿茶酚的培养基中,漆酶的活性为1.22 U ml -1,比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下,漆酶活性略有下降,最大活性为1.1 Uml -1。结果表明,在以儿茶酚为碳源的培养基中,灰杉漆酶活性高于葡萄糖。漆酶的研究主要集中在担子菌科的木腐真菌中,特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明,青苔漆酶在酸性pH范围内具有活性,最适pH为4.5。在含10 gL -1儿茶酚的培养基中,漆酶的活性为1.22 U ml -1,比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下,漆酶活性略有下降,最大活性为1.1 Uml -1。结果表明,在以儿茶酚为碳源的培养基中,灰杉漆酶活性高于葡萄糖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Lipoxygenase Inhibitory Activities of Some Plant Extracts and Chemical Compounds Three wheat cultivars’ responses to NaCl treatments: changes in lipid peroxidation, cell viability, hydrogen peroxide content and antioxidant defence parameters The role of Escherichia coli bacterioferritin in stressed induced conditions Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria Scale Surface Microstructure and Scale Size in Three Mugilid Fishes (Teleostei, Mugilidae) of Iran from Three Different Habitats
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1