{"title":"Some kinetic properties of amylase from the intestine of the rabbitfish, Siganus canaliculatus (Park)","authors":"Uma Sabapathy, L.H. Teo","doi":"10.1016/0305-0491(94)90150-3","DOIUrl":null,"url":null,"abstract":"<div><p>The optimum pH of the amylase was 6.5 at 30, 37, 40 and 50°C. At pH 6.5, the amylase had an optimum temperature of 25°C and activation energy (<em>E</em><sub>a</sub>) of 7.3 kcal/mol. The thermostability of the enzyme was determined at 45 and 50°C. The <em>K</em><sub>m</sub> values were determined at various temperatures and found to be lowest at 10 and 50°C and highest at 20°C.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 1","pages":"Pages 139-144"},"PeriodicalIF":0.0000,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90150-3","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0305049194901503","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
The optimum pH of the amylase was 6.5 at 30, 37, 40 and 50°C. At pH 6.5, the amylase had an optimum temperature of 25°C and activation energy (Ea) of 7.3 kcal/mol. The thermostability of the enzyme was determined at 45 and 50°C. The Km values were determined at various temperatures and found to be lowest at 10 and 50°C and highest at 20°C.
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