{"title":"Radiation-induced organogenesis : effects of irradiated medium and its components on tobacco tissue culture","authors":"N. Degani","doi":"10.1016/0033-7560(75)90007-1","DOIUrl":null,"url":null,"abstract":"<div><p>Gamma irradiated medium induces the formation of buds in non-irradiated dark grown tobacco callus (<em>Nicotiana tabacum</em> Var. Wisconsin no. 38). Experiments were conducted to determine the component(s) of the medium that is effective in this radiation-induced organogenesis. Fractions of medium were irradiated singly and in combination, then combined with non-irradiated fractions to form the complete growth medium.</p><p>The results show that irradiated indoleacetic acid (IAA) was not the effective component in the induction of organogenesis. Omission of IAA from the medium resulted in the formation of buds, as expected. Irradiated myo-inositol induced organogenesis more consistently than the other irradiated components.</p><p>The age of the inoculum tissue and its passage number from the tobacco stem affected the potency of the tobacco callus to organise.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"15 4","pages":"Pages 363-366"},"PeriodicalIF":0.0000,"publicationDate":"1975-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0033-7560(75)90007-1","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation Botany","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0033756075900071","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
Gamma irradiated medium induces the formation of buds in non-irradiated dark grown tobacco callus (Nicotiana tabacum Var. Wisconsin no. 38). Experiments were conducted to determine the component(s) of the medium that is effective in this radiation-induced organogenesis. Fractions of medium were irradiated singly and in combination, then combined with non-irradiated fractions to form the complete growth medium.
The results show that irradiated indoleacetic acid (IAA) was not the effective component in the induction of organogenesis. Omission of IAA from the medium resulted in the formation of buds, as expected. Irradiated myo-inositol induced organogenesis more consistently than the other irradiated components.
The age of the inoculum tissue and its passage number from the tobacco stem affected the potency of the tobacco callus to organise.
γ辐照培养基诱导未辐照的暗生烟草愈伤组织(Nicotiana tabacum Var. Wisconsin no.)萌发芽。38)。进行了实验以确定在这种辐射诱导的器官发生中有效的培养基成分。将部分培养基单独辐照和组合辐照，然后与未辐照的部分相结合，形成完整的生长培养基。结果表明，辐照的吲哚乙酸(IAA)不是诱导器官发生的有效成分。正如预期的那样，培养基中缺少IAA导致了芽的形成。与其他辐照成分相比，辐照肌醇诱导的器官发生更为一致。接种组织的年龄及其离茎传代数影响烟草愈伤组织的组织能力。
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