Induction of Hairy Roots in Cyclobalanopsis glauca and Production of Catechin in Hairy Roots

Abstract

Cyclobalanopsis glauca (Thunb.) Oerst is a tree species with rich polyphenol compounds, including catechins, which increase the oxidation resistance of blood plasma and reduce the risk of cardiovascular disease in humans. Catechin may also have an inhibitory effect on carcinogens, specifically those related to skin and lung cancer. This study investigated hairy root induction in leaves of C. glauca infected with 2 strains of Agrobacterium rhizogenes (BCRC15010 and BCRC15785) under the influence of α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). After 45 d, explants co-cultivated with the BCRC15785 strain in MS medium with 0.1 mg L^(-1) NAA and 300 mg L^(-1) cefotaxime exhibited 32.5% greater efficiency of hairy root induction. Induction of calli and hairy roots was observed under the influence of NAA, while only calli were induced under the influence of 2,4-D. We observed the direct influence of the infection period on the transformation frequency, and it increased as the infection period increased up to 24 h. Extracts from hairy roots, calli, and natural seedling roots revealed the presence of catechin content, and concentrations were 4.203, 3.413, and 4.668 mg g^(-1), respectively, in these 3 analytes.