Characterization of auxin production plant growth promotion by a bacterium isolated from button mushroom compost

Jisu Yoo, Heon-Hak Lee, Chang-Hoon Han, Min-ho Yoon
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引用次数: 1

Abstract

An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof 94.3 mg L-1 was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at 35oC. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.
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从蘑菇堆肥中分离的一种细菌对生长素产生植物生长促进作用的研究
从忠南保宁市废蘑菇堆肥中分离到一株生长素产菌,编号为4-3。根据化学分类和系统发育分析,菌株4-3被归类为一株新菌株。分离得到的L. tardus菌株4-3产生长素吲哚-3-乙酸(IAA)。在含0.1% l-色氨酸的R2A培养基中,35℃孵育24 h,最大IAA产量为94.3 mg L-1。IAA产量与培养基pH呈负相关,表明IAA的增加引起了培养基的酸化。还评估了补充不同浓度的l-色氨酸(已知的IAA前体)的效果。浓度为0.1%时产量最大,浓度超过0.2%时产量下降。为研究该菌对绿豆的促生作用,采用4-3菌株培养液接种绿豆水培养物和种盆。结果表明,不定根诱导率和根生长率是未接种植株的2.2倍。
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