The Effects of High-Intensity Interval Training on Skeletal Muscle Morphological Changes and Denervation Gene expression of Aged Rats

IF 1.3 Q3 SPORT SCIENCES Montenegrin Journal of Sports Science and Medicine Pub Date : 2019-09-01 DOI:10.26773/MJSSM.190906
S. Tayebi, M. Siahkouhian, M. Keshavarz, M. Yousefi
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引用次数: 3

Abstract

Muscle denervation is one of the most critical pathological factors involved in muscle atrophy as a result of ageing. This study aims to investigate the chronic effect of high-intensity interval training (HIIT) on cross-sectional areas and muscle denervation genes, interpreted in the plantaris muscle atrophy of aged rats. Twenty-eight aged and young rats were divided into four different groups, including exercise and control. The training protocol included six weeks of HIIT. Animals were sacrificed 48 hours after the last training session, and the plantaris muscle was removed. To measure Gadd45a mRNA and NCAM1mRNA, we used a real-time PCR technique. The cross-sectional area was measured with photomyography using an H&E technique. The results showed that ageing significantly decreased NCAM1 mRNA in the aged control group (p=0.0001) and exercise leads to a significant increase (interaction effect) of it (p=0.003). Gadd45a mRNA was significantly increased due to ageing (p=0.009), and exercise resulted in a significant decrease in that in the aged exercise group (p=0.04). The cross-sectional area in the aged control group was significantly smaller than in the young control group (p=0.046). In contrast to young groups, exercise could increase the cross-sectional area in the aged exercise group compared with the aged control group, but it was not significant (p=0.069). It seems that HIIT could improve ageing-induced muscular atrophy, although denervation-involved gene modification leads to an increase in the muscular cross-sectional area; therefore, it improves muscular atrophy due to ageing.
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高强度间歇训练对老年大鼠骨骼肌形态变化及去神经支配基因表达的影响
肌肉失神经支配是衰老引起肌肉萎缩的重要病理因素之一。本研究旨在探讨高强度间歇训练(HIIT)对老年大鼠足底肌萎缩的横截面积和肌肉去神经支配基因的慢性影响。28只年老和年轻的大鼠被分成四个不同的组,包括运动组和对照组。训练方案包括6周的HIIT。动物在最后一次训练后48小时被处死,跖肌被切除。为了测量Gadd45a mRNA和NCAM1mRNA,我们使用了实时PCR技术。采用H&E技术用光度法测量横截面积。结果表明,衰老显著降低老龄对照组NCAM1 mRNA表达(p=0.0001),运动显著增加NCAM1 mRNA表达(交互作用)(p=0.003)。衰老导致Gadd45a mRNA显著升高(p=0.009),运动导致老年运动组Gadd45a mRNA显著降低(p=0.04)。老年对照组的横截面积明显小于年轻对照组(p=0.046)。与年轻组相比,运动可使老年运动组的横截面积比老年对照组增加,但差异不显著(p=0.069)。HIIT似乎可以改善衰老引起的肌肉萎缩,尽管去神经支配相关的基因修饰导致肌肉横截面积增加;因此,它可以改善因衰老引起的肌肉萎缩。
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来源期刊
CiteScore
2.80
自引率
0.00%
发文量
14
审稿时长
9 weeks
期刊介绍: MJSSM covers all aspects of sports science and medicine; all clinical aspects of exercise, health, and sport; exercise physiology and biophysical investigation of sports performance; sport biomechanics; sports nutrition; rehabilitation, physiotherapy; sports psychology; sport pedagogy, sport history, sport philosophy, sport sociology, sport management; and all aspects of scientific support of the sports coaches from the natural, social and humanistic side.
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