Antioxidant, Lipo-Protective and Antibacterial Activities of Phytoconstituents Present in Solanum Xanthocarpum Root

International Review of Biophysical Chemistry Pub Date : 2012-07-01 DOI:10.15866/IREBIC.V3I3.1557

A. Pandey, Shashank Kumar

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引用次数: 56

Abstract

Present study reports the biochemical and antibacterial activities of phytoconstituents present in roots of non toxic weed S. xanthocarpum. Total phenolic contents were quantified in different fractions. Ethanolic (ET) fraction was richest (194.44±0.41 mgPGE/g) followed by ethyl acetate (EA) and aqueous (AQ) fractions (115±0.25 and 88.75± mgPGE/g, respectively). Antioxidant activity was evaluated using reducing power assay (RPA) while lipo-protective activity was determined by lipid peroxidation inhibition (LPOI)/TBARS assays in rat kidney tissue. ET, AQ and HX (hexane) fractions demonstrated significant reducing power at 1000 µg/ml concentration showing dose dependent response in the range 200-1000 µg/ml. ET and AQ fractions exhibited 52% and 45% LPOI, respectively in TBARS assay. Positive correlation was observed between total phenol and RPA (r2=0.399) as well as %LPOI (r2=0.540). Similarly relationship between RPA and %LPOI was also found to be positive (r2=0.379). Pathogenic bacteria Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus exhibited resistance against AQ fraction in disc diffusion assay

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龙葵根中植物成分的抗氧化、脂质保护和抗菌活性研究

本研究报道了无毒杂草黄杉(S. xanthocarpum)根中植物成分的生化活性和抗菌活性。测定不同馏分中总酚含量。乙醇(ET)组分含量最高(194.44±0.41 mgPGE/g)，其次是乙酸乙酯(EA)和水(AQ)组分(分别为115±0.25和88.75±mgPGE/g)。采用还原力法(RPA)评价大鼠肾组织的抗氧化活性，采用脂质过氧化抑制法(LPOI)/TBARS法测定其脂质保护活性。ET, AQ和HX(己烷)馏分在1000µg/ml浓度下表现出显著的还原能力，在200-1000µg/ml范围内表现出剂量依赖性。在TBARS试验中，ET和AQ组分的LPOI分别为52%和45%。总酚与RPA (r2=0.399)、%LPOI (r2=0.540)呈正相关。RPA与%LPOI之间也存在类似的正相关关系(r2=0.379)。病原菌大肠埃希菌、粪肠球菌、铜绿假单胞菌、肺炎克雷伯菌和金黄色葡萄球菌对AQ组分表现出耐药性

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International Review of Biophysical Chemistry

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