Pengaruh Penyimpanan dan Lama Pemanasan Terhadap Kadar Asam Galat pada Kacang Tanah (Arachis hypogaea L.)

Devyana Dyah wulandari, Ersalina Nidianti, Ary Andini, Rahmawati Fitri Awalia, Halimah Prisilia
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Abstract

One of the most prevalent phenolic acids to be found in foods such as fruits, vegetables, and herbal remedies is gallic acid, also known as 3,4,5-trihydroxybenzoic acid. The free acids, esters, catechin derivatives, and hydrolyzed tannins are the different ways that these substances are obtained. The specialty of this compound is due to its pharmacological activity as a radical scavenger. The total phenolic content in peanuts varies from 132.5 to 248.8 mg GAE/100 g. The purpose of this study was to determine the effect of storage time and heating time of peanuts (Arachis hypogaea L.) on gallic acid levels. Determination of gallic acid using the High Performance Liquid Chromatography (HPLC) method with the column used is an ODS (Octadesil Silica) C18 column and the mobile phase is a mixture of acetonitrile and aquadest (1:3). Peanuts were stored for 1 week, 2 weeks, and 3 weeks at 25°C and fried at 160°C for 2 minutes, 4 minutes, and 6 minutes. The results of this study on the length of storage obtained a P value of 0.000 where the results are smaller than 0.05. This indicates that storage time has a significant effect on gallic acid levels in peanut samples. Gallic acid levels appear to increase from the first week to the fourth week. Based on the results of the research at the time of heating obtained a P value of 0.189 where the results are higher than 0.05. This shows that the length of heating time does not affect on the gallic acid content in the peanut samples. From these results, it was concluded that gallic acid content was affected by storage time, but not by heating time.
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在水果、蔬菜和草药等食物中发现的最普遍的酚酸之一是没食子酸,也被称为3,4,5-三羟基苯甲酸。游离酸、酯、儿茶素衍生物和水解单宁是获得这些物质的不同方式。这种化合物的特殊之处在于其作为自由基清除剂的药理活性。花生中总酚含量为132.5 ~ 248.8 mg GAE/100 g。本研究的目的是确定花生(arachhis hypogaea L.)储存时间和加热时间对没食子酸水平的影响。采用高效液相色谱法测定没食子酸,色谱柱为ODS (Octadesil Silica) C18,流动相为乙腈与aquadest(1:3)的混合物。花生在25℃下保存1周、2周和3周,在160℃下煎炸2分钟、4分钟和6分钟。本研究对贮藏长度的研究结果P值为0.000,其中P值小于0.05。这表明储藏时间对花生样品中没食子酸含量有显著影响。没食子酸水平从第一周到第四周增加。根据加热时的研究结果得出P值为0.189,其中P值高于0.05。这说明加热时间的长短对花生样品中没食子酸的含量没有影响。结果表明,没食子酸含量受贮藏时间的影响,而不受加热时间的影响。
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