Nitric Oxide Inhibitory Activity of Crude Extracts and Extraction Time and Temperature Optimization of Pectin from Cissampelos pareira Leaves

Abstract

This research studied crude extracts of Cissampelos pareira leaves using fi ve solvents as hexane, dichloromethane, ethyl acetate, methanol, and ethanol for nitric oxide (NO) inhibitory activity and maximize pectin yields from facile green extraction. Five crude hexane, dichloromethane, ethyl acetate, methanol and ethanol extracts were obtained from the leaves of C. pareira at 0.4%, 0.5%, 0.3%, 3%, and 1%, respectively. The hexane, dichloromethane, and ethyl acetate extracts showed the most potent decrease in NO production with half-maximal inhibitory concentration (IC50) values of 49.85±5.07, 56.26±1.49, and 64.55±2.37 (μg/mL), respectively. Concentrations of all crude extracts of C. pareira indicated no cellular toxicity. Pectin was aqueously extracted at 40 to 90 °C for 15 to 120 min and yields and quality were examined. Highest pectin yield (36.12%) was obtained after 60 min at 60 °C, with quality meeting the criteria established by the International Pectin Producers Association (IPPA) and categorized as high methoxyl pectin.