Identification Of Lipase – Producing Psychrophilic Yeast, Leucosporidium Sp.

F. Rashid, R. Rahim, D. Ibrahim
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引用次数: 6

Abstract

Cold-active enzymes have recently received great attention due to their potential applications in a broad range of industrial, agricultural and medical processes. One of the enzymes is lipase (triacyglycerol acylhydrolases E.C 3.1.1.3) which is unique in catalyzing the hydrolysis of triacylglycerols into free fatty acids and glycerol. In this particular research, an obligate psychrophilic microorganism was isolated from Casey Station, Antarctica. The growth of this microorganism has been tested at different temperatures, 4C, 27C and 37C. At 4C, the microorganism was able to grow whereas at 27C and 37C, there was no growth at all. The presence of lipase enzyme in this microorganism was detected by halo zone on palm oil (substrate) agar plates. Identification of this microorganism was done based on its morphological, biochemical and molecular characteristics. For the morphology analysis, two types of microscopy observation were carried out: phase contrast microscopy and Scanning Electron Microscopy (SEM). Both observations showed budding structures. This suggested that this particular microorganism is psychrophilic yeast. Biochemical tests were done based on its capability to ferment and assimilate sugar. In addition, assimilation of nitrate was also tested. In molecular approach, the genomic DNA (gDNA) of this microorganism was successfully extracted and the extracted gDNA was used for amplification via polymerase chain reaction (PCR) technique using Internal Transcribed Spacer (ITS) primers. The PCR product obtained was sequenced and submitted for Basic Local Alignment Search Tool (BLAST) at National Center for Biotechnological Information (NCBI). The analysis showed that this microorganism contained ITS sequences (highest identity with Leocosporidium sp.).
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产脂肪酶嗜冷酵母的鉴定。
由于冷活性酶在工业、农业和医疗过程中具有广泛的应用前景,近年来受到了广泛的关注。其中一种酶是脂肪酶(三酰基甘油酰基水解酶E.C 3.1.1.3),它是催化三酰基甘油水解成游离脂肪酸和甘油的独特酶。在这项特殊的研究中,从南极洲的凯西站分离出一种专性的嗜冷微生物。在4℃、27℃和37℃的不同温度下,对这种微生物的生长进行了测试。在4℃时,微生物能够生长,而在27℃和37℃时,微生物根本没有生长。在棕榈油(底物)琼脂板上用晕带法检测该微生物中脂肪酶的存在。根据其形态、生化和分子特征对其进行鉴定。形态学分析采用相衬显微镜和扫描电镜两种显微观察方法。两次观测都显示出出芽结构。这表明这种特殊的微生物是嗜冷酵母。根据其发酵和吸收糖的能力进行了生化试验。此外,还测试了硝酸盐的同化作用。在分子方法上,成功提取了该微生物的基因组DNA (gDNA),并利用内部转录间隔物(ITS)引物通过聚合酶链反应(PCR)技术进行扩增。对所得PCR产物进行测序,并提交给美国国家生物技术信息中心(NCBI)的基本局部比对检索工具(BLAST)。分析结果表明,该微生物含有ITS序列,与Leocosporidium sp.同源性最高。
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