Enzyme Activity as an Indicator of Soil Quality in Sterile Waste Heap

A. M. Dodocioiu, C. Babeanu, D. Buzatu
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Abstract

Abstract Recent research reports that enzymes accumulated in soil participate in the cycles of elements and contribute to soil fertility. The purpose of this paper is to determine the enzymatic activity and the humus content in the materials from waste heap in order to assess their fertility and to establish possible re-cultivation technologies. The investigations were carried out on materials from the Pesteana North waste heap. In order to achieve this, the activity of catalase, invertase, urease and phosphatase activity was determined in different plots of the heap during the summer and as a control the zonal soil was used. Enzymatic activities were colorimetric assay using appropriate substrates. The obtained results show a large variation in the values of the determined enzymatic activities. In all investigated plots, all enzymes are found to be lower than those determined for the zonal soil. A strong positive correlation is observed between enzymatic activities of catalase and invertase and the accumulation of humus which demonstrates that enzymatic activities has a useful tool for studying the evolution of heap materials to agricultural soils.
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酶活性作为无菌废物堆土壤质量的指标
近年来的研究表明,土壤中积累的酶参与元素循环,对土壤肥力有贡献。本文的目的是测定废物堆中材料的酶活性和腐殖质含量,以评估其肥力并建立可能的再培养技术。调查是对来自Pesteana North废物堆的材料进行的。为了实现这一目标,在夏季测定了堆中不同地块的过氧化氢酶、转化酶、脲酶和磷酸酶活性,并使用地带性土壤作为对照。采用合适底物比色法测定酶活性。所得结果表明,测定的酶活性值变化很大。在所有调查样地中,发现所有酶都低于地带性土壤的测定值。过氧化氢酶和转化酶活性与腐殖质积累呈显著正相关,表明酶活性是研究堆料向农业土壤演化的有效工具。
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