{"title":"Isolation of Facultative Anaerobic Bacterial Pathogens from Canned Food and Use of Lactobacillus Plantarum as A Bio-Control Agent","authors":"Khudija Malik, Hussan Ibne Shoukani, Sabayyel Hassan, Saima Bibi, S. Bano","doi":"10.54393/pbmj.v6i08.911","DOIUrl":null,"url":null,"abstract":"Preserved foods can play a significant role in causing food poisoning when they are not handled, processed, or stored properly. Objective: To investigate facultative anaerobic foodborne bacterial pathogens from canned foods and to control their growth Lactobacillus plantarum was used as a bio-control agent. Methods: Different canned food samples were collected to isolate and identify facultative anaerobic bacterial pathogens. Results: Out of n=65 samples, n=13 samples cultured positive as facultative anaerobes. They were further confirmed with biochemical and molecular identifications as foodborne bacterial pathogens with a ratio of 62% Escherichia coli, 30% Salmonella typhimurium, and 8% Vibrio cholerae. During bio-control studies, the results revealed possible inhibition of facultative anaerobic bacterial pathogens by using purified compounds of Lactobacillus plantarum. Conclusions: The use of probiotics in canned foods requires careful consideration, as factors such as the specific strain, food matrix, processing conditions, and storage practices can influence its effectiveness.","PeriodicalId":19844,"journal":{"name":"Pakistan BioMedical Journal","volume":"3 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan BioMedical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54393/pbmj.v6i08.911","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Preserved foods can play a significant role in causing food poisoning when they are not handled, processed, or stored properly. Objective: To investigate facultative anaerobic foodborne bacterial pathogens from canned foods and to control their growth Lactobacillus plantarum was used as a bio-control agent. Methods: Different canned food samples were collected to isolate and identify facultative anaerobic bacterial pathogens. Results: Out of n=65 samples, n=13 samples cultured positive as facultative anaerobes. They were further confirmed with biochemical and molecular identifications as foodborne bacterial pathogens with a ratio of 62% Escherichia coli, 30% Salmonella typhimurium, and 8% Vibrio cholerae. During bio-control studies, the results revealed possible inhibition of facultative anaerobic bacterial pathogens by using purified compounds of Lactobacillus plantarum. Conclusions: The use of probiotics in canned foods requires careful consideration, as factors such as the specific strain, food matrix, processing conditions, and storage practices can influence its effectiveness.