Isolation of Facultative Anaerobic Bacterial Pathogens from Canned Food and Use of Lactobacillus Plantarum as A Bio-Control Agent

Khudija Malik, Hussan Ibne Shoukani, Sabayyel Hassan, Saima Bibi, S. Bano
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Abstract

Preserved foods can play a significant role in causing food poisoning when they are not handled, processed, or stored properly. Objective: To investigate facultative anaerobic foodborne bacterial pathogens from canned foods and to control their growth Lactobacillus plantarum was used as a bio-control agent. Methods: Different canned food samples were collected to isolate and identify facultative anaerobic bacterial pathogens. Results: Out of n=65 samples, n=13 samples cultured positive as facultative anaerobes. They were further confirmed with biochemical and molecular identifications as foodborne bacterial pathogens with a ratio of 62% Escherichia coli, 30% Salmonella typhimurium, and 8% Vibrio cholerae. During bio-control studies, the results revealed possible inhibition of facultative anaerobic bacterial pathogens by using purified compounds of Lactobacillus plantarum. Conclusions: The use of probiotics in canned foods requires careful consideration, as factors such as the specific strain, food matrix, processing conditions, and storage practices can influence its effectiveness.
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罐头食品兼性厌氧病原菌的分离及植物乳杆菌的生物防治应用
腌制食品如果处理、加工或储存不当,会在引起食物中毒方面发挥重要作用。目的:研究罐头食品中兼性厌氧食源性致病菌,并对其生长进行控制。方法:采集不同罐头食品样品,分离鉴定兼性厌氧菌致病菌。结果:65份样品中,兼性厌氧菌培养阳性13份。经生化和分子鉴定证实为食源性致病菌,大肠杆菌占62%,鼠伤寒沙门菌占30%,霍乱弧菌占8%。在生物防治研究中,结果显示植物乳杆菌纯化化合物可能抑制兼性厌氧细菌病原体。结论:在罐头食品中使用益生菌需要慎重考虑,因为特定菌株、食品基质、加工条件和储存方式等因素都会影响其有效性。
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