Effect of Probiotics Bifidobacterium lactis And Lactobacillus casei on SpermParameters and Expression of Apoptosis BAX and Bcl2 Genes in Streptozotocin-Induced Diabetic Rats

Abstract

Diabetes mellitus is a metabolic disorder, which leading to male reproductive disorders. Probiotic bacteria are known to be one of the most effective factors in many diseases. This study aimed to evaluate the effect of probiotics Bifidobacterium lactis and lactobacillus casei on sperm parameters and expression of apoptosis BAX and Bcl2 genes in streptozotocin-induced diabetic rats. In this experimental study, 35 adult male Wistar rats were divided into five control groups, diabetic, diabetic treated with: B.lactis, L.casei and both probiotic B.lactis and L.casei. Diabetic was induced by intraperitoneal injection of streptozotocin at a dose of 60 mg/Kg and Probiotic treatment at a dose of 109 cfu/ml for 56 days was done by gavage. One day after the last gavage, blood glucose, serum insulin, sperm parameters and histology and Histomorphometric of testicular tissue were examined. Also, the total amount of RNA was extracted from the treated testicular tissue and analyzed by Real-time PCR. The data’s were evaluated using one-way ANOVA and Tukey, p-value test less than 0.05. In this study, the reduction of sperm parameters, insulin serum levels, Spermatogenesis coefficient and increased blood glucose, Spermatogenesis coefficient testicular tissue degradation was observed significantly in the diabetic group compared to the control group (P<0.001). However, in the treated groups with probiotics, a decrease in blood glucose, an increase in sperm parameters, insulin levels and a decrease in testicular tissue damage was observed in comparison with the diabetic group (P<0.05). Also, the expression of BAX and Bcl2 genes in diabetic groups showed a significant change compared to the control group (P<0.05), but there were no significant changes in probiotic treated groups compared with diabetes. This study demonstrates the effect of improving probiotics receiving diabetic groups on the damage parameters sperm and testicular tissue in diabetic rats. Probiotics may eventually secrete and produce antioxidant and anti-inflammatory compounds and reduce the damaging effects of diabetes.