Peculiarities of pigment complex functioning of winter wheat plants depending on the fertilizer application method

Z. Bilousova, V. Keneva, Y. Klipakova
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Abstract

To obtain the maximum yield of winter wheat, it is necessary to further optimize the existing cultivation technologies in the direction of their adaptation to changing environmental conditions. One of the areas of adaptation of plants to adverse abiotic factors is the active functioning of the photosynthetic apparatus, which depends on the amount of nutrients introduced. The influence of fertilizer application on the condition of the pigment complex of winter wheat plants in the conditions of the Southern Steppe of Ukraine has been studied. Two varieties of winter wheat were selected for the study: Shestopalivka and Mason. The experiment scheme involved the application of fertilizers at sowing (K0; K12) and foliar treatment with various tank mixtures (urea; urea + magnesium sulfate; urea + magnesium sulfate + potassium monophosphate). The pigment content has been determined by grinding fresh leaves of winter wheat, followed by the addition of a solvent in the form of acetone. Measurements of pigments were performed using a spectrophotometer. According to the research results, it was established that before the foliar treatment the a-chlorophyll content and carotenoids was higher in the plant leaves of the Shestopalivka variety. At the same time, the b-chlorophyll content on the contrary was higher for plants of the Mason variety by 17%, which may be due to the adaptation of plants of this variety to lack of light. On the 3rd day after foliar treatment, a decrease in the pigment content in the plant leaves of all experimental variants has been observed, which was due to the active growth of the photosynthetic surface and a decrease in the total dry matter mass. There was no significant difference between the varieties of the content of photosynthetic pigments in this period. On the 10th day after foliar treatment, an increase in a- and b-chlorophyll content has been observed for both studied varieties, which may be the result of adaptation of the photosynthetic apparatus of winter wheat plants to lighting conditions. Foliar treatment of winter wheat plants with a tank mixture of urea with magnesium sulfate and potassium monophosphate contributed to a further increase in the content of a-chlorophyll by 12-23%, and b-chlorophyll by 5-37% depending on the variety compared to the control. The results of the research testify to the high efficiency of complex application of nitrogen-phosphorus-potassium fertilizers for foliar treatment of winter wheat plants in the BBCH 31 stage, both against the background of pre-sowing application of potassium fertilizers and without it.
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施肥方式对冬小麦色素复合体功能的影响
为了获得冬小麦的最大产量,有必要进一步优化现有的栽培技术,使其适应不断变化的环境条件。植物适应不利的非生物因子的一个领域是光合机构的积极功能,这取决于引入的营养物质的量。在乌克兰南部草原条件下,研究了施肥对冬小麦色素复合体状况的影响。研究选择了两个冬小麦品种:Shestopalivka和Mason。试验方案为播种时施肥(K0;K12)和叶面处理用各种槽混合物(尿素;尿素+硫酸镁;尿素+硫酸镁+单磷酸钾)。通过研磨冬小麦鲜叶,然后加入丙酮溶剂来测定色素的含量。用分光光度计对色素进行测定。研究结果表明,在叶面处理前,该品种植物叶片中a-叶绿素和类胡萝卜素含量较高。与此同时,梅森品种的b-叶绿素含量反而高出17%,这可能是由于该品种对光照不足的适应所致。在叶片处理后第3天,所有试验变异体植物叶片色素含量均下降,这是由于光合表面的活跃生长和总干物质质量的减少。这一时期各品种间光合色素含量无显著差异。在叶片处理后第10天,两个品种的a-和b-叶绿素含量均有所增加,这可能是冬小麦植株光合机构适应光照条件的结果。与对照相比,尿素与硫酸镁和一磷酸钾混合处理的冬小麦叶片,a-叶绿素含量进一步增加了12-23%,b-叶绿素含量进一步增加了5-37%,具体取决于品种。本研究结果表明,在播前施钾和不施钾的情况下,bbch31期冬小麦叶片复合施氮磷钾均具有较高的效率。
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