Study on Bio-film forming bacterial pathogens in diabetic foot ulcer

Abstract

Actinomycetes due to their unique repertoire of antimicrobial secondary metabolites can be a good source to control human pathogens. In our research plan, soil samples were collected from Bhilai, India. The strains were isolated using yeast extract-malt extract agar medium and identified based on their morphological, physiological, and biochemical characteristics. The antifungal activity of isolates was examined by the perpendicular streak plate as well as the agar well diffusion method. Out of 14 isolates, only 4 isolates (28.5%) showed antifungal activity. Further, one isolate showed the highest antifungal activity and was identified as Streptomyces antibioticus A8 based on PIB-Win software and the identification score was 0.99. Antifungal activity of new isolate Streptomyces antibioticus A8 against the T. rubrum MTCC 296, with a zone of inhibition 28±0.0 mm, whereas minimum activity was recorded against A. niger MTCC 872 with the zone of inhibition 9±0.0 mm. antifungal activity against C.albicans ATCC10231, C.albicans ATCC90028, C. albicans ATCC24433, C.albicans MTCC183, C.tropicalis MTCC184, A.alternata MTCC 1779 was recorded as 20±0.0mm, 17±0.0 mm, 17±0.0 mm, 15±0.5 mm, 14±0.0 mm and 11±0.0 mm respectively by agar well diffusion method. Further, discovering new antimicrobial-producingmicrobes probably will be helpful for uncovering novel therapeutic agents against a broad range of pathogenic organisms.