Influence Of Deubiquitinating Enzymes On Mutagenesis In Saccharomyces Cerevisiae

J. Gong, W. Siede
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引用次数: 2

Abstract

In recent years, it became clear that the mutagenic effect of base alterations in a DNA template is dependent on bypass synthesis, carried out by one or more translesion polymerases. A critical role for proliferating cell nuclear antigen (PCNA) and its ubiquitination following DNA damage has been established. Among Saccharomyces cerevisiae deletion mutants in which UV mutagenesis is compromised, we identified and characterized a mutant of the ubiquitin recycling protein Doa4. Similar cases may be represented by Doa1, previously described by others, as well as Bro1 and Ubi4. We discuss overall altered ubiquitin levels or failure to deubiquitinate specific target proteins as likely explanations. This study is of relevance for understanding and possibly modifying the mutagenic effect of DNA-damaging agents in environmental toxicology, cancer treatment and cancer prevention.
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脱泛素酶对酿酒酵母诱变的影响
近年来,人们越来越清楚,DNA模板中碱基改变的致突变作用依赖于由一个或多个翻译聚合酶进行的旁路合成。DNA损伤后增殖细胞核抗原(PCNA)及其泛素化的关键作用已经确立。在紫外线诱变受损的酿酒酵母缺失突变体中,我们鉴定并鉴定了泛素回收蛋白Doa4的突变体。类似的情况可以由Doa1,以及Bro1和Ubi4代表,之前被其他人描述过。我们讨论了泛素水平的总体改变或特异性靶蛋白去泛素化失败作为可能的解释。本研究对了解dna损伤剂在环境毒理学、癌症治疗和癌症预防等领域的致突变作用并进行可能的修饰具有重要意义。
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