Activity of rosemary (Rosmarinus officinalis - Family: Lamiaceae) essential oil compared to peracetic acid in Listeria monocytogenes biofilms

Q3 Agricultural and Biological Sciences Acta Scientiarum. Biological Sciences Pub Date : 2023-06-15 DOI:10.4025/actascibiolsci.v45i1.67277

Emanoelli Aparecida Rodrigues dos Santos, L. E. Tadielo, Thiago Henrique Bellé, Jhennifer Arruda Schmiedt, V. H. C. Dias, Paulo Henrique Silva Orisio, C. K. Cerqueira-Cézar, J. G. Pereira, V. C. Barcellos, L. Bersot

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Abstract

The objective of this study was to evaluate the activity of Rosmarinus officinalis essential oil (EO) compared to peracetic acid (PA) regarding formation and elimination of Listeria monocytogenes biofilms on polystyrene surface. The minimum inhibitory concentration (MIC) was determined according to standard protocol. Isolates were inoculated according to MIC standards polystyrene plate wells, which were then incubated at 37°C/96 hours for evaluation of biofilm formation. Regarding the evaluation of biofilm elimination, the biofilms were treated under MIC for 10 minutes. The MIC obtained were 2.0 and 3.0 mg mL-1 for EO and 0,015% for PA. Therefore, the results showed a reduction in the formation of biofilm with the presence of EO and PA, EO being more efficient (p < 0.05). Both compounds had a good capacity of eliminating biofilms, however the EO reduced the biofilm formation when compared to PA, highlighting its potential as an antibacterial agent and antibiofilm.

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迷迭香精油与过氧乙酸在单核增生李斯特菌生物膜中的活性比较

本研究的目的是评价迷迭香精油(EO)与过氧乙酸(PA)在聚苯乙烯表面形成和消除单核细胞增生李斯特菌生物膜的活性。根据标准方案测定最低抑菌浓度(MIC)。分离株按MIC标准聚苯乙烯板孔接种，37℃/96小时孵育，评价生物膜形成情况。对生物膜的去除效果进行评价，生物膜在MIC下处理10分钟。EO的MIC分别为2.0和3.0 mg mL-1, PA为0.015%。因此，结果表明，EO和PA的存在减少了生物膜的形成，EO效率更高(p < 0.05)。两种化合物均具有良好的去除生物膜的能力，但与PA相比，EO减少了生物膜的形成，突出了其作为抗菌剂和抗生物膜的潜力。

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来源期刊

Acta Scientiarum. Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

38 weeks

期刊介绍： The journal publishes original articles in all areas of Biological Sciences, including anatomy, bacteriology, molecular biology, biochemistry, botany, cytology and cell biology, animal behavior, ecology, limnology, embryology, and histology, morpho-physiology, genetics, microbiology, parasitology and zoology.