Erythrocyte Immobilisation with Total Protein Extracted from Gnetum gnemon L. Seeds on Variety of Solid Media

Abstract

The objective of this research was to determine the effectiveness of erythrocyte immobilisation with total protein extracted from gnetum seeds on a variety of solid media, i.e., filter papers, doff plastics, and nitrocellulose membranes. Old gnetum seeds were ground to make powders. The powders were homogenized in ml PBS buffer containing anti-protease PMSF in a cool room. The homogenate was centrifuged in order to separate supernatant from pellet. Solid media were punched with a perforator, so the results were discs of papers, plastics, and nitrocellulose membranes. The solid media perforated discs were then dipped in gnetum total protein supernatants. The dipped discs were air-dried for a while. Human erythrocytes of all ABO groups were washed three times and diluted to suspension of 20% (v/v) in NaCl 0,9% (w/v). A small drop of erythrocyte suspension was put in middle of each solid media disc, and its blood suspension was spread evenly. Next, the disc was observed under an USB digital microscope to see whether there was erythrocyte immobilisation on solid media coated with total proteins of gnetum seeds or not. All the erythrocytes from human ABO groups were bound effectively, from strongest to weakest,to paper filters, nitrocellulose membranes, and plastics. The future use of erythrocyte immobilisation on a variety of solid media coated with of gnetum total protein seed may be useful for diagnosing diseases related patological erythrocytes with a specific marker.