A redox hydrogel integrated PQQ–glucose dehydrogenase based glucose electrode

M. Alkasrawi, I. Popescu, B. Mattiasson, E. Csöregi, V. Laurinavicius
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引用次数: 17

Abstract

The present work describes the design and characteristics of a glucose electrode based on a newly isolated and purified, oxygen-independent, pyrrole quinoline quinone glucose dehydrogenase (PQQ–GDH) integrated in a redox hydrogel (poly(1-vinylimidazole) complexed with Os(4-4′-dimethylbipyridine)2Cl) using poly(ethylene glycol)diglycidyl ether (PEGDGE) as a crosslinker. The influence of the buffer’s nature and the presence of PQQ and Ca2+ ions on the enzyme stability were considered. The characteristics of the optimal electrode design were compared to those obtained for similarly prepared, glucose oxidase based electrodes. The optimal electrode configuration inserted in a single manifold flow injection system, was characterised by a linear range up to 100 µM, a sensitivity of 5.9 mA M–1 cm–2, and a detection limit of 5 µM (calculated as three times the signal-to-noise ratio).
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一种氧化还原水凝胶集成pq -葡萄糖脱氢酶葡萄糖电极
本工作描述了一种以聚乙二醇二甘油酯醚(PEGDGE)为交联剂,将新分离纯化的氧不依赖的吡咯喹啉醌葡萄糖脱氢酶(PQQ-GDH)整合在氧化还原水凝胶(聚(1-乙烯基咪唑)与Os(4-4′-二甲基联吡啶)2Cl络合)中的葡萄糖电极的设计和特性。考虑了缓冲液性质、PQQ和Ca2+离子的存在对酶稳定性的影响。将最佳电极设计的特征与类似制备的葡萄糖氧化酶电极的特征进行了比较。最佳电极配置插入到单流形注射系统中,其特点是线性范围高达100µM,灵敏度为5.9 mA M - 1 cm-2,检测限为5µM(计算为信噪比的三倍)。
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