Method Validation and Measurement Uncertainty (MU) Evaluation on Enrofloxacin and Ciprofloxacin in the Aquatic Products.

Abstract

This study aimed to investigate a detection method of enrofloxacin and ciprofloxacin to be avail for strictly supervising the quality and safety of aquatic products. The results displayed that the optimal extraction conditions for enrofloxacin and ciprofloxacin were the following five aspects: 15 g dosages of Na₂SO₄ to dehydrate, 8‰ of acetonitrile and 50% hydrochloric acid to deproteinization, 2 mL dosages of n-hexane to degrease, 10 min of ultrasonic time, and 20 min of extraction (stand) time. Meanwhile, it was also obtained for the optimal detection performance indexes of the recovery, precision, and accuracy from the tests of shrimp, grass carp, and tilapia. In particular, the expanded uncertainties were 2.8601 and 0.8613, and the factors of both the calibration curves (U_rel(C)) and the analysis of the experiment (U_rel(E)) were the two MU main contributors for enrofloxacin and ciprofloxacin together with the results above 40%. Consequently, the developed novel method was suited for the determination of the enrofloxacin and ciprofloxacin residues in aquatic products and would contribute to reinforce in supervision and inspection of the quality and safety of aquatic products.