INVESTIGATION OF CHANGES IN SOME BIOACTIVE PROPERTIES OF PHENOLIC EXTRACTS FROM PULP AND SEED TISSUES OF ZIZIPHUS JUJUBA DURING IN VITRO DIGESTION

Eda Şensu, A. Duran, B. Özçelik, Aysun Yücetepe
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Abstract

In this study, changes in angiotensin-I converting enzyme (ACE) inhibitory, α-amylase inhibitory and antioxidant activities, total phenolic content (TPC), total monomeric anthocyanin content (TMAC) of ultrasonic phenolic extracts from pulp and seed of Ziziphus jujuba were investigated during in vitro digestion. Bioaccessible fractions of phenolics in seed and pulp extracts were calculated as 23.24±4.46% and 9.43±0.24%, respectively. Moreover, bioaccessibility for TMAC in seed extracts (147.83±9.20%) was higher than pulp (15.76±3.89%) (P<0.05). A decrease in the antioxidant activity of the extracts occurred after in vitro digestion (P<0.05). The ACE inhibitory activity of undigested extracts from seed (86.04±0.00%) was higher than that of the undigested pulp extract (42.74±8.57%) (P<0.05). The α-amylase inhibitory activity of seed and pulp extracts was determined as 49.18±0.35% and 36.07±5.83%, respectively. The results of the study showed that ACE inhibitory activity and α-amylase inhibitory activity of the polyphenolics from pulp increased after in vitro digestion.
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红枣果肉和种子组织酚类提取物体外消化过程中部分生物活性特性变化的研究
研究了红枣果肉和种子超声酚类提取物体外消化过程中血管紧张素- 1转换酶(ACE)抑制、α-淀粉酶抑制和抗氧化活性、总酚含量(TPC)、总单体花青素含量(TMAC)的变化。种子和果肉提取物中酚类物质的生物可达性分别为23.24±4.46%和9.43±0.24%。种子提取物TMAC的生物可及性(147.83±9.20%)高于果肉(15.76±3.89%)(P<0.05)。体外消化后,提取物的抗氧化活性降低(P<0.05)。未消化种子提取物的ACE抑制活性(86.04±0.00%)高于未消化果肉提取物(42.74±8.57%)(P<0.05)。种子提取物和果肉提取物的α-淀粉酶抑制活性分别为49.18±0.35%和36.07±5.83%。结果表明,体外消化后,果肉多酚类物质的ACE抑制活性和α-淀粉酶抑制活性均有所提高。
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