Valérie Pineau Noël, Maxence Larose, Quentin Perry-Auger, P. De Koninck, D. Côté
{"title":"Speckled illumination HiLo microscopy for fast calcium imaging of zebrafish brain","authors":"Valérie Pineau Noël, Maxence Larose, Quentin Perry-Auger, P. De Koninck, D. Côté","doi":"10.1109/PN52152.2021.9598001","DOIUrl":null,"url":null,"abstract":"Studying the cellular activity of the brain requires good spatial and temporal resolutions and most importantly optical sectioning, but current imaging systems can become quite expensive, need special care or do not have all the required properties. In addition, the FOV is reduced to reach reasonable acquisition speed, which prevents whole-brain imaging. Here, we present HiLo microscopy to acquire volumetric activity of the zebrafish brain in vivo. This project focuses on improving our knowledge of the brain-gut-microbiota axis in a living organism.","PeriodicalId":6789,"journal":{"name":"2021 Photonics North (PN)","volume":"1 1","pages":"1-1"},"PeriodicalIF":0.0000,"publicationDate":"2021-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2021 Photonics North (PN)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/PN52152.2021.9598001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Studying the cellular activity of the brain requires good spatial and temporal resolutions and most importantly optical sectioning, but current imaging systems can become quite expensive, need special care or do not have all the required properties. In addition, the FOV is reduced to reach reasonable acquisition speed, which prevents whole-brain imaging. Here, we present HiLo microscopy to acquire volumetric activity of the zebrafish brain in vivo. This project focuses on improving our knowledge of the brain-gut-microbiota axis in a living organism.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
