A Vitrification Method by Means of a Straw to Prevent Infections in Mouse Pronuclear Embryos

M. Kumon, Y. Kumasako, T. Utsunomiya, Y. Araki
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引用次数: 4

Abstract

Mouse p ronuc lear em bryos we re cryopreserved by a simple and safe vitrification method. In the process, Vitrification Media VT101, Thawing Media VT 102 (KITAZATO. Co. Japan) and the embryos were loaded into a straw; then they were cryopreserved. Different loading methods were examined to determine the safety levels of crystallization for the embryo’s survival after thawing. The best condition attained, after thawing, was a 75% embryo survived rate of which 66% developed to the two-cell stage, 71% developed to the morula stage and 27% developed to the blastosyst stage. This development of embryos after vitrification was not significantly different to that of a control group without freezing and thawing. The vitrification method was considered to protect embryos against various infections via liquid nitrogen during cryopreservation. It is expected that the method can be applied to human embryos.
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用吸管玻璃化法预防小鼠原核胚胎感染
采用一种简单、安全的玻璃化冷冻方法保存小鼠原核胚胎。在此过程中,玻璃化介质VT101,解冻介质vt102 (KITAZATO)。将胚胎装入吸管;然后冷冻保存。研究了不同的加载方法,以确定解冻后胚胎存活的结晶安全水平。解冻后获得的最佳条件是胚胎存活率为75%,其中66%发育为二细胞期,71%发育为桑葚胚期,27%发育为囊胚期。玻璃化后胚胎的发育与未冷冻和解冻的对照组没有显著差异。玻璃化方法被认为是在冷冻保存过程中通过液氮保护胚胎免受各种感染的方法。该方法有望应用于人类胚胎。
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