Optimization of explants, media, plant growth regulators and carbohydrates on callus induction and plant regeneration in Citrus jambhiri Lush.

IF 0.2 Q4 HORTICULTURE Journal of Horticultural Sciences Pub Date : 2023-06-30 DOI:10.24154/jhs.v18i1.2159
G. S. Sidhu, P. Malhotra, H. S. Rattanpal
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Abstract

Callus induction was attempted from the four explants viz. root, cotyledon, epicotyl, and leaf segments excised from in vitro raised seedlings of C. jambhiri. Among various MS media supplementations with growth regulators and carbohydrates, the maximum (95.50%) and the earliest (8.30 days) callogenesis was obtained in epicotyl segments, when cultured on MS medium supplemented with NAA (10.0 mgl-1) + BAP (1.0 mgl-1) + sucrose (8%). The modified MS (macro and micro-nutrients reduced to half) fortified with BAP (5.0 mgl-1) + GA3 (3.0 mgl-1) recorded maximum shoot regeneration (43.10%) from callus, with an average of 5.30 shoots per callus after 35.50 days of culturing. However, prolonged exposure to GA3 resulted in thin elongated shoots and leaves. The age of the callus substantially influenced the plant regeneration frequency. The potency of the callus to regenerate decreased significantly with an increase in the age of the callus. Shoot regeneration was recorded maximum (43.43%) in 60 days old calli, followed by 90 days old (30.48%) calli, whereas it was minimum (10.46%) in 150 days old calli. The maximum (79.50%) shoot proliferation was recorded in MS medium supplemented with BAP (1.0 mgl-1) + Kin (0.5 mgl-1) with an average of 5.06 shoots per culture. The MS medium fortified with NAA (1.0 mgl-1) + IBA (1.0 mgl-1) induced maximum (77.33%) rooting, with an average of 3.19 roots per shoot after 13.4 days of culturing. Rooted plants were hardened and survived the best (83.6%) on the potting mixture consisting of cocopeat + vermiculite + perlite (2:1:1).
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外植体、培养基、植物生长调节剂和碳水化合物对柑桔愈伤组织诱导和植株再生的影响。
以离体培养的蚕豆根、子叶、上胚轴和叶段为外植体,尝试诱导愈伤组织。在添加生长调节剂和碳水化合物的MS培养基中,在添加NAA (10.0 mg -1) + BAP (1.0 mg -1) +蔗糖(8%)的MS培养基中,上胚轴部分的胼胝质形成最多(95.50%),最早(8.30 d)。添加BAP (5.0 mg -1) + GA3 (3.0 mg -1)的改良MS(宏微量营养素减少一半)的愈伤组织再生率最高(43.10%),培养35.50 d后平均每个愈伤组织再生5.30个芽。然而,长时间暴露于GA3会导致茎和叶变细变长。愈伤组织年龄对植株再生频率有显著影响。愈伤组织再生能力随愈伤组织年龄的增加而显著下降。60日龄愈伤组织的茎再生率最高(43.43%),90日龄愈伤组织次之(30.48%),150日龄愈伤组织的茎再生率最低(10.46%)。在添加BAP (1.0 mg -1) + Kin (0.5 mg -1)的MS培养基中,芽增殖率最高(79.50%),平均每次培养5.06个芽。NAA (1.0 mg -1) + IBA (1.0 mg -1)的MS培养基生根率最高,为77.33%,培养13.4 d后平均每枝生根量为3.19根。生根植株在椰泥+蛭石+珍珠岩(2:1:1)的盆栽混合物中硬化效果最好,成活率为83.6%。
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来源期刊
Journal of Horticultural Sciences
Journal of Horticultural Sciences Agricultural and Biological Sciences-Plant Science
CiteScore
0.30
自引率
0.00%
发文量
0
审稿时长
6 weeks
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