Marina Indriasari, Hardinsyah, L. Kustiyah, B. Priosoeryanto, F. H. Idris
{"title":"Determination of the Dose of Erythrosine B as a Preparation for Inducing the Experimental Rats to Be Spastic","authors":"Marina Indriasari, Hardinsyah, L. Kustiyah, B. Priosoeryanto, F. H. Idris","doi":"10.22606/MCMR.2019.32001","DOIUrl":null,"url":null,"abstract":"This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.","PeriodicalId":91930,"journal":{"name":"Modern clinical medicine research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Modern clinical medicine research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22606/MCMR.2019.32001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.
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