Colorimetric sensor for label free detection of porcine PCR product

Abstract

Selective detection of specific DNA sequences is increasingly getting momentum in clinical diagnosis, pathology, genetics [1–3] and food analysis [4, 5]. The polymerase chain reaction (PCR) is a commonly used technique to amplify specific sequence segment in nearly all DNA-based assays [3]. The use of PCR addresses both the sensitivity issues and sample purification steps producing a large quantity DNA from as little as single copy. However, post- PCR analysis of PCR amplified DNA involves complex and time consuming electrophoresis, blot analysis or sequencing [3].