M. Bandehpour, T. Nafarieh, Fatemeh Zahedipour, A. Tavasoli, B. Kazemi
{"title":"CRISPR-Cas9 Mediated Capsule Gene Silencing in Escherichia coli","authors":"M. Bandehpour, T. Nafarieh, Fatemeh Zahedipour, A. Tavasoli, B. Kazemi","doi":"10.22037/NBM.V6I4.21672","DOIUrl":null,"url":null,"abstract":"Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli. M aterials and Methods: We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay. R es ults: The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria. Conclusion: E. coli without any capsule around may proper for replacement of it with other molecules in future.","PeriodicalId":19372,"journal":{"name":"Novelty in Biomedicine","volume":"39 1","pages":"162-166"},"PeriodicalIF":0.0000,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Novelty in Biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22037/NBM.V6I4.21672","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli. M aterials and Methods: We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay. R es ults: The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria. Conclusion: E. coli without any capsule around may proper for replacement of it with other molecules in future.
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