Antioxidant Activities of Extracts from Different Parts of Sasa borealis

Jun-wei Kang, J. Chang, Ji-Hyun Yoo, Eun-Soo Doh, K. Kil
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引用次数: 5

Abstract

Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were 24.6 mg/ml and 14.3 mg/ml, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.
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北方莎草不同部位提取物的抗氧化活性研究
目的:研究北花莎草叶、茎、根的水提物和乙醇提取物的抗氧化活性。方法:用水和乙醇制备白沙叶、茎、根提取物。分析了总多酚和总黄酮的含量。以2,2-二苯基-1-吡啶肼基(DPPH)自由基清除活性、SOD样活性、2,2'-氮基-双(3-乙基苯并噻唑-6-磺酸)(ABTS)自由基清除活性、羟基自由基清除活性和亚硝酸盐清除活性测定其抗氧化活性。结果:乙醇提取物的抗氧化活性明显高于水提取物。叶乙醇提取物的总多酚和总黄酮含量分别为24.6 mg/ml和14.3 mg/ml,远高于其他部位。SOD样活性以70%乙醇提取物最高,达15.68%。70%乙醇提取物的给电子能力最高,为59.07%。其给电子能力高于BHT(45.68%)。在pH为2.5和pH为4.2时,70%乙醇提取物的亚硝酸盐清除活性高于水提取物。亚硝酸盐清除活性以70%乙醇提取物最高,达到75.2%。清除羟基自由基的活性以70%乙醇提取物最高,为16.16%,低于BHT(61.56%)。结论:70%乙醇提取物具有较强的抗氧化活性,可作为天然抗氧化剂。
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