Pub Date : 2021-01-01DOI: 10.6116/KJH.2021.36.1.41
Gyuyou Noh, S. Byun, Jong Rok Lee, Sook-Jahr Park, Sang Chan Kim
{"title":"Protective effect of Citrus unshiu peel on the cadmium-induced apoptosis in HepG2 cells","authors":"Gyuyou Noh, S. Byun, Jong Rok Lee, Sook-Jahr Park, Sang Chan Kim","doi":"10.6116/KJH.2021.36.1.41","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.1.41","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"54 1","pages":"41-49"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84744763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.6116/KJH.2021.36.3.15
Minhee Kim, Myunghoon Kang, Jeong Hoon Lee, K. Leem, Hyo-Jin An, Jong-Sik Jin, Jong-Hyun Lee, Jae-Ki Chang, Shin Seong, Wonnam Kim
Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). *Corresponding author : Wonnam Kim, Cnh Center for Cancer Research, Gangnam-gu, Seoul 06154, Republic of Korea. ·Tel : +82-70-7711-8529 ·Fax : +82-70-8282-7007 ·E-mail : eb75lab@gmail.com #First author : Minhee Kim, Division of Pharmacology, College of Korean Medicine, Semyung University, Jecheon, 27136, Republic of Korea. ·E-mail : mickeymhk@gmail.com Myunghoon Kang, Division of Pharmacology, College of Korean Medicine, Semyung University, Jecheon, 27136, Republic of Korea. ·E-mail : kangmh1994@naver.com Jeonghoon Lee, Herbal Crop Research Division, NIHHS, RDA, Eumseong, Chungbuk, 27709, Republic of Korea. ·E-mail : artemisia@korea.kr ·Received : 12 April 2021 ·Revised : 28 April 2021 ·Accepted : 25 May 2021 16 大 韓 本 草 學 會 誌 ― Vol. 36 No. 3, 2021 Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 μg/ml). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.
目的:由韩国农村振兴厅园艺草药研究所通过甘草与乌拉甘草的种间杂交,培育出甘草新品种WONGAM和SINWONGAM。本研究比较了甘草新品种(WONGAM和SINWONGAM)与官方纲目(glycyrhiza glabra和glycyrhiza uralensis)的抗氧化和细胞毒作用。*通讯作者:Wonnam Kim, Cnh癌症研究中心,首尔江南区,06154,大韩民国。·电话:+82-70-7711-8529·传真:+82-70-8282-7007·E-mail: eb75lab@gmail.com #第一作者:Minhee Kim, Semyung大学韩国医学院药学系,Jecheon, 27136,大韩民国。·E-mail: mickeymhk@gmail.com世明大学韩国医学院药物科姜明勋(myunhoon Kang,音),仁川市27136。·E-mail: kangmh1994@naver.com韩国国立卫生研究院、RDA草药作物研究部,韩国忠北阴城27709。·e-邮件:artemisia@korea.kr·收稿日期:2021年4月12日·修订日期:2021年4月28日·接受日期:2021年5月25日16中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:中文:MTT法测定细胞毒性,倒置显微镜观察细胞形态。结果:测定了不同浓度(0、10、50、100、500、1000 μg/ml)甘草、乌拉尔甘草、WONGAM和SINWONGAM对DPPH、ABTS、亚硝酸盐自由基的清除能力和还原能力。甘草、乌拉尔甘草、WONGAM和SINWONGAM的抗氧化活性表现出相似的剂量依赖性。甘草新品种和官方纲目对HCT116、HT29、A549、MDA-MB231、PC3、ACHN和HeLa细胞的细胞毒作用随剂量增加无显著差异。甘草、乌拉甘草、WONGAM和SINWONGAM对AGS、MCF7和Hep3B细胞的细胞毒性有显著差异。结论:甘草新品种及官方纲目具有较强的抗氧化作用。此外,发现等效的细胞毒性效力观察到在细胞依赖的方式。我们的研究表明,甘草新品种可能提供各种各样的健康益处。
{"title":"Antioxidant Activity and Cytotoxicity against Human Cancer Cells of Glycyrrhiza New Varieties : A Comparison with Glycyrrhiza Official Compendia","authors":"Minhee Kim, Myunghoon Kang, Jeong Hoon Lee, K. Leem, Hyo-Jin An, Jong-Sik Jin, Jong-Hyun Lee, Jae-Ki Chang, Shin Seong, Wonnam Kim","doi":"10.6116/KJH.2021.36.3.15","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.3.15","url":null,"abstract":"Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). *Corresponding author : Wonnam Kim, Cnh Center for Cancer Research, Gangnam-gu, Seoul 06154, Republic of Korea. ·Tel : +82-70-7711-8529 ·Fax : +82-70-8282-7007 ·E-mail : eb75lab@gmail.com #First author : Minhee Kim, Division of Pharmacology, College of Korean Medicine, Semyung University, Jecheon, 27136, Republic of Korea. ·E-mail : mickeymhk@gmail.com Myunghoon Kang, Division of Pharmacology, College of Korean Medicine, Semyung University, Jecheon, 27136, Republic of Korea. ·E-mail : kangmh1994@naver.com Jeonghoon Lee, Herbal Crop Research Division, NIHHS, RDA, Eumseong, Chungbuk, 27709, Republic of Korea. ·E-mail : artemisia@korea.kr ·Received : 12 April 2021 ·Revised : 28 April 2021 ·Accepted : 25 May 2021 16 大 韓 本 草 學 會 誌 ― Vol. 36 No. 3, 2021 Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 μg/ml). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"28 1","pages":"15-24"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91315622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.6116/KJH.2021.36.3.39
Youngsoo Choi, Eun-Mi Kim, Seong Hee Lee, Hyosang Han, K. Kim
{"title":"Investigation on the effect of water extracts of Mangifera indica leaves on the hair loss-related genes in human dermal papilla cells","authors":"Youngsoo Choi, Eun-Mi Kim, Seong Hee Lee, Hyosang Han, K. Kim","doi":"10.6116/KJH.2021.36.3.39","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.3.39","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"13 1","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86563721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pureum Noh, W. Kim, Inkyu Park, Sungyu Yang, G. Choi, B. Moon
{"title":"Development of ITS sequence based SCAR marker and multiplex-SCAR assay for the rapid authentication of Tetrapanacis Medulla and Akebiae Caulis","authors":"Pureum Noh, W. Kim, Inkyu Park, Sungyu Yang, G. Choi, B. Moon","doi":"10.6116/KJH.2021.36.1.9","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.1.9","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"33 1","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88538336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gyuyou Noh, Jong Rok Lee, J. K. Kim, Sang Mi Park, Sook-Jahr Park, Sang Chan Kim
{"title":"Protective effect of Citri Unshius Pericarpium against cadmium-induced liver damage in mice","authors":"Gyuyou Noh, Jong Rok Lee, J. K. Kim, Sang Mi Park, Sook-Jahr Park, Sang Chan Kim","doi":"10.6116/KJH.2021.36.1.1","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.1.1","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"21 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86372922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.6116/KJH.2021.36.1.97
Da-eun Kang, NamJoon Cho, G. Renchinkhand, B. Lee, Eun-Mi Kim, M. Nam, K. Kim
{"title":"Effects of Liriope muscari Water Extracts on the Cell Death and Inflammatory Cytokine Expression of Poly I:C-treated Lung Carcinoma Cells","authors":"Da-eun Kang, NamJoon Cho, G. Renchinkhand, B. Lee, Eun-Mi Kim, M. Nam, K. Kim","doi":"10.6116/KJH.2021.36.1.97","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.1.97","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"1 1","pages":"97-102"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79772663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.6116/KJH.2021.36.1.67
Myoung-Jin Kim, Hye-Min Choi, Byung-Woo Yu, Y. Hong, Chae-Suk Ra, Min-ju Kim, Jung Ok Kim
{"title":"Development of tablets and evaluation of ingredient content and pharmacological effects of Yukgunja-tang","authors":"Myoung-Jin Kim, Hye-Min Choi, Byung-Woo Yu, Y. Hong, Chae-Suk Ra, Min-ju Kim, Jung Ok Kim","doi":"10.6116/KJH.2021.36.1.67","DOIUrl":"https://doi.org/10.6116/KJH.2021.36.1.67","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"6 2","pages":"67-76"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91491895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hosong Cho, Boyoung Lee, Wonkyung Lee, J. H. Lee, D. Park, Changil Choi, M. Jin, S. Roh, Young-sung Ju
{"title":"The effects of a Mixture of Puerariae Flos Extract and Citri Unshius Pericarpium Extract on Estrogenic Activities and Osteoclastogenesis","authors":"Hosong Cho, Boyoung Lee, Wonkyung Lee, J. H. Lee, D. Park, Changil Choi, M. Jin, S. Roh, Young-sung Ju","doi":"10.6116/KJH.2020.35.3.1","DOIUrl":"https://doi.org/10.6116/KJH.2020.35.3.1","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"64 11 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77042869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.6116/KJH.2020.35.5.13
Yun-Mi Kang, Wonnam Kim, Jong-Sik Jin, Jonghyun Lee, Jae-Ki Chang, J. Lee, Hyo-Jin An
{"title":"The Comparative Study of Anti-allergic Effect by Glycyrrhiza New Varieties and Official Compendia","authors":"Yun-Mi Kang, Wonnam Kim, Jong-Sik Jin, Jonghyun Lee, Jae-Ki Chang, J. Lee, Hyo-Jin An","doi":"10.6116/KJH.2020.35.5.13","DOIUrl":"https://doi.org/10.6116/KJH.2020.35.5.13","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"81 1","pages":"13-21"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73542807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}